Core--Mouse maintenance and typing

该中心包括一个中央繁殖和分型设施，以协调中心研究人员使用的小鼠的维护和分配。在这个设施中饲养的老鼠是明确定义的。转基因小鼠的维持对该中心至关重要，6个项目中有5个使用转基因小鼠，因此对这样一个核心的需求是合理的。因此，在中心内维持一个转基因小鼠核心是合适的。应用程序中描述的小鼠活动水平证明雇用某人来管理菌落并对后代进行分类（特别是在产生新的小鼠系时）是合理的。不幸的是，根据所提供的信息，很难估计在给定的时间内需要对多少只老鼠进行基因分型，比较医学工作人员已经提供了哪些服务作为他们每日费用的一部分（例如，菌落管理是否包括在内？），或者雇用这样的职位在多大程度上减少了每日费用。然而，考虑到Core同时维持单转基因和双转基因小鼠，集中筛选是一个谨慎的举动。这将确保质量（基因型的有效性）和中心成员的小鼠可用性。

This core consists of a central breeding and typing facility to coordinate the maintenance and distribution of mice used by investigators of the Center. The mice to be housed in this facility are explicitly defined. The maintenance of transgenic mice is critical to this Center, and the need for such a core is well justified with 5 of the 6 projects utilizing genetically modified mice. Therefore, it is appropriate to maintain a transgenic mouse Core within the Center. The level of mouse activity described in the application justifies the hiring of someone to manage the colonies and type offspring (particularly once new lines of mice are being generated). Unfortunately, with the information provided, it is difficult to estimate how many mice need to be genotyped over a given period, what services are already provided by the Comparative Medicine staff as part of their per diem charges (e.g. is colony management part of that charge?), or to what extent the hiring of such a position reduces per diem charges. Nevertheless, given that the Core is maintaining both single and double transgenic mice, the centralization of screening is a prudent move. This will ensure quality (validity of genotypes) and availability of mice to the members of the Center.