Core--Mouse maintenance and typing

这一核心包括一个中央育种和打字设施，以协调中心调查人员使用的小鼠的维护和分发。这个设施中要安置的老鼠是明确定义的。转基因小鼠的维护对该中心至关重要，6个利用转基因小鼠的项目中有5个项目充分证明了对这样一个核心的需求。因此，在该中心内保持转基因小鼠核心是合适的。应用程序中描述的老鼠活动水平证明了雇佣某人管理群体和后代类型是合理的(特别是在新的老鼠品系产生的情况下)。不幸的是，根据所提供的信息，很难估计在给定的时期内有多少小鼠需要进行基因分型，比较医学工作人员已经提供了哪些服务作为其每日费用的一部分(例如，群体管理是该费用的一部分吗？)，或者雇用这样的职位在多大程度上减少了每天的费用。然而，鉴于Core同时保留了单转基因和双转基因小鼠，集中筛选是一项谨慎的举措。这将确保小鼠的质量(基因类型的有效性)和可供中心成员使用。

This core consists of a central breeding and typing facility to coordinate the maintenance and distribution of mice used by investigators of the Center. The mice to be housed in this facility are explicitly defined. The maintenance of transgenic mice is critical to this Center, and the need for such a core is well justified with 5 of the 6 projects utilizing genetically modified mice. Therefore, it is appropriate to maintain a transgenic mouse Core within the Center. The level of mouse activity described in the application justifies the hiring of someone to manage the colonies and type offspring (particularly once new lines of mice are being generated). Unfortunately, with the information provided, it is difficult to estimate how many mice need to be genotyped over a given period, what services are already provided by the Comparative Medicine staff as part of their per diem charges (e.g. is colony management part of that charge?), or to what extent the hiring of such a position reduces per diem charges. Nevertheless, given that the Core is maintaining both single and double transgenic mice, the centralization of screening is a prudent move. This will ensure quality (validity of genotypes) and availability of mice to the members of the Center.