CORE 2: Maintaining Genetically Engineered Mice for Collaborations

Silvio O.麻省理工学院的康特神经科学研究中心（CCNR）需要基因工程小鼠。此外，这些小鼠中的许多被提议在涉及两个、三个甚至四个实验室的协作安排中生成和/或分析。这些小鼠的另一个特征是，它们中的许多都是条件性工程改造的，因此需要两种或三种组成品系的杂交， 是模块化的（例如，Cre、floxed、tTA和Otet小鼠）。许多这些组成小鼠在不同的组合中杂交，用于多个合作项目的不同特定目标。因此，我们建议在麻省理工学院与其比较医学系合作建立这些组成小鼠的中央繁殖群，该系负责监督和服务麻省理工学院的整个动物设施。中心菌落用于维持各种菌株，并偶尔向各个实验室提供这些菌株。在个别项目中描述的特定实验中使用的小鼠杂交的费用将由个别教师的预算收取。在索尔克研究所的海涅曼实验室中生产、维护和使用的突变和转基因小鼠将由他个人的预算支付。产生核心#1和单个项目中所述菌株的成本将计入相应的预算。只有在组件小鼠建立后，它们才被转移到核心#2进行维护。MIT将提供鼠标放置空间。比较医学部的工作人员将提供标准的维护服务、换笼、垫料、食物、水和健康监测，核心将支付每日费用。在第一年，将保留约15至20个菌株，我们预计在随后几年将增加约10个菌株，因为它们是新生产的。由于多个项目的大量需求，预计一些集落（如fNR 1、CA 1-Cre和CA 3-Cre）将相对较大（50笼），而其他集落（如偶尔用作对照的全局NR 1 KO、全局GluR 6 KO和CA 3-NR 1 KO小鼠）将保持较小（10笼）。该核心提供的服务包括：（1）维护菌落，（2）对个体小鼠进行基因分型，以及（3）将小鼠运送到索尔克研究所和其他外部合作者。核心#2将允许向中心内的合作实验室（有时在中心外）提供保持相同的小鼠品系。

Many Individual Projects of the proposed Silvio O. Conte Center for Neuroscience Research (CCNR) at MIT require genetically engineered mice. Furthermore, many of these mice are proposed to be generated and/or analyzed in collaborative arrangements involving two, three or even four laboratories. Another characteristic feature of these mice is that many of them are conditionally engineered and, therefore, require crossing of two or three component strains that are modular (e.g., Cre, floxed, tTA and Otet mice). Many of these component mice are crossed in different combinations for different specific aims across multiple collaborative projects. Therefore, we propose to set up central breeding colonies of these component mice at MIT in collaboration with its Department of Comparative Medicine which supervises and services MIT's entire animal facility. The central colonies are meant to be for the maintenance of the various strains and occasional supplying of these strains to individual laboratories. The cost for the intercrossing of mice to be used in specific experiments described in the Individual Projects will be charged to the individual faculty's budget. The mutant and transgenic mice to be produced, maintained, and used in Heinemann's lab at the Salk Institute will be charged to his individual budget. The cost to generate the strains described in Core #1 and individual projects will be charged to the corresponding budgets. Only after the component mice are established will they be transferred to Core #2 for maintenance. The mouse holding space will be provided by MIT. The standard maintenance services, cage changes, bedding, food, water, and health monitoring will be provided by the staff of the Department of Comparative Medicine for which the Core will pay per diem charges. In Year 1 about 15 to 20 strains will be maintained and we expect that about 10 strains will be added in subsequent years as they are newly made. It is expected that some colonies such as fNR1, CA1-Cre, and CA3-Cre, will be relatively large (50 cages) because of the heavy demands from multiple projects while others such as global NR1 KO, global GluR6 KO, and CA3-NR1 KO mice to be used occasionally as a control will be kept small (10 cages). The service provided by this Core consists of (1) maintenance of the colonies, (2) genetic typing of individual mice, and (3) shipment of mice to the Salk Institute and other outside collaborators. Core #2 will permit supplies of identically maintained mouse strains to the collaborating labs within the Center and sometimes beyond the Center.