CORE 2: Maintaining Genetically Engineered Mice for Collaborations

麻省理工学院拟议中的西尔维奥·O·孔特神经科学研究中心(CCNR)的许多个人项目需要基因工程小鼠。此外，建议在涉及两个、三个甚至四个实验室的协作安排中产生和/或分析其中许多小鼠。这些小鼠的另一个特征是，它们中的许多是有条件的工程，因此需要两到三个组成品系的杂交， 是模块化的(例如，Cre、Floated、Tta和Otet小鼠)。这些组件中的许多鼠标以不同的组合进行杂交，以实现跨多个协作项目的不同特定目标。因此，我们建议与麻省理工学院比较医学系合作，在麻省理工学院建立这些成分小鼠的中央繁育群体，该部门负责监督和服务麻省理工学院的整个动物设施。中心菌落用于维持各种菌株，并偶尔将这些菌株提供给个别实验室。在个别项目中描述的特定实验中使用的小鼠交叉杂交的费用将计入个别教员的预算中。海尼曼在索尔克研究所的实验室将生产、维护和使用突变和转基因小鼠，这些小鼠的费用将由他个人预算支付。产生核心#1和个别项目所述菌株的费用将计入相应的预算。只有在组件鼠标建立后，它们才会被转移到核心#2进行维护。鼠标放置空间将由麻省理工学院提供。标准维护服务、笼子更换、床上用品、食物、水和健康监测将由比较医学系的工作人员提供，核心将按日支付费用。在第一年，将保留大约15至20个菌株，我们预计在随后几年将增加大约10个菌株，因为它们是新制造的。由于多个项目的繁重需求，预计一些群体，如fNR1、CA1-Cre和CA3-Cre，将相对较大(50个笼子)，而其他一些群体，如全球NR1 KO、全球GluR6 KO和偶尔用作对照的CA3-NR1KO小鼠将保持较小的规模(10个笼子)。该中心提供的服务包括(1)维持群体，(2)对单个小鼠进行基因分型，以及(3)将小鼠运送给索尔克研究所和其他外部合作者。核心2号将允许向该中心内的合作实验室提供相同维护的小鼠品系，有时还可以提供给该中心以外的实验室。

Many Individual Projects of the proposed Silvio O. Conte Center for Neuroscience Research (CCNR) at MIT require genetically engineered mice. Furthermore, many of these mice are proposed to be generated and/or analyzed in collaborative arrangements involving two, three or even four laboratories. Another characteristic feature of these mice is that many of them are conditionally engineered and, therefore, require crossing of two or three component strains that are modular (e.g., Cre, floxed, tTA and Otet mice). Many of these component mice are crossed in different combinations for different specific aims across multiple collaborative projects. Therefore, we propose to set up central breeding colonies of these component mice at MIT in collaboration with its Department of Comparative Medicine which supervises and services MIT's entire animal facility. The central colonies are meant to be for the maintenance of the various strains and occasional supplying of these strains to individual laboratories. The cost for the intercrossing of mice to be used in specific experiments described in the Individual Projects will be charged to the individual faculty's budget. The mutant and transgenic mice to be produced, maintained, and used in Heinemann's lab at the Salk Institute will be charged to his individual budget. The cost to generate the strains described in Core #1 and individual projects will be charged to the corresponding budgets. Only after the component mice are established will they be transferred to Core #2 for maintenance. The mouse holding space will be provided by MIT. The standard maintenance services, cage changes, bedding, food, water, and health monitoring will be provided by the staff of the Department of Comparative Medicine for which the Core will pay per diem charges. In Year 1 about 15 to 20 strains will be maintained and we expect that about 10 strains will be added in subsequent years as they are newly made. It is expected that some colonies such as fNR1, CA1-Cre, and CA3-Cre, will be relatively large (50 cages) because of the heavy demands from multiple projects while others such as global NR1 KO, global GluR6 KO, and CA3-NR1 KO mice to be used occasionally as a control will be kept small (10 cages). The service provided by this Core consists of (1) maintenance of the colonies, (2) genetic typing of individual mice, and (3) shipment of mice to the Salk Institute and other outside collaborators. Core #2 will permit supplies of identically maintained mouse strains to the collaborating labs within the Center and sometimes beyond the Center.