Core--Mouse physiology

本核心的目标是提供转基因小鼠的有效生理特性，以帮助与该SCOR的协同RO1应用相关的多学科研究项目。允许组织特异性操作的基因靶向和转基因技术的出现迅速将基础心血管研究的重点从研究细胞和亚细胞系统转移到整个器官。随着遗传知识和技术在小鼠中的发展，以及研究小鼠器官生理学的小型化系统越来越多，该物种已成为心血管生理学、病理学和发育生物学的良好模型系统。由于转基因大鼠、兔子和猪的产生比最初预期的要困难、耗时和昂贵得多，可以预见，小鼠模型系统在心血管病理生理中的重要性将在未来进一步增加。贝斯以色列女执事医疗中心心血管部致力于成为专注于小鼠心脏分子生物学和生理学的领先研究中心之一。因此，我们建立了小鼠生理学核心实验室，以提供集中的服务和培训设施，以确保RFA的每位参与研究者能够为他们的研究表征转基因和基因靶向小鼠。小鼠生理学核心提供了最先进的诊断，以表征小鼠心血管生理学，包括分离的心脏制剂与Ca2+检测，血流动力学测量，心电图和心律失常监测。提议的核心将实现该RFA的多学科研究目标，而传统的RO1框架无法解决这些目标。

The goal of this Core is to provide efficient physiological characterization of transgenic mice in order to aid multi-disciplinary research projects relevant to the collaborative RO1 applications for this SCOR. The advent of gene-targeting and transgenic-techniques allowing for tissue-specific manipulations has rapidly shifted the focus of basic cardiovascular research from studying cellular and subcellular systems to the whole organ. With genetic knowledge and technologies being most advanced in the mouse, and miniaturized systems to study murine organ physiology becoming increasingly available, this species has emerged as the favorable model system for cardiovascular physiology, pathology, and developmental biology. Because the generation of transgenic rats, rabbits and pigs has turned out to be much more difficult, time consuming, and expensive than initially expected, it can be predicted that the importance of mouse model systems in cardiovascular pathophysiology will further increase in the future. The Cardiovascular Division of Beth Israel Deaconess Medical Center has made a major commitment to become one of the leading research centers focusing on the murine cardiac molecular biology and physiology. Accordingly, we have established the Mouse Physiology Core Laboratory to provide centralized service and training facilities that will ensure the ability of each participating investigator of the RFA to characterize transgenic and gene-targeted mice for their research. The Mouse Physiology Core provides state-of-the-art diagnostics for characterizing murine cardiovascular physiology, including isolated heart preparations with Ca2+ detection, hemodynamics measurements, electrocardiograms and arrhythmia monitoring. The proposed Core will enable accomplishment of multi-disciplinary research goals of this RFA that cannot be addressed by a traditional RO1 framework.