Core--Mouse physiology

该核心的目标是提供转基因小鼠的有效生理特征，以帮助与该SCOR的协作RO1应用相关的多学科研究项目。基因打靶和转基因技术的出现，使得心血管基础研究的重点迅速从细胞和亚细胞系统转移到整个器官。随着小鼠遗传知识和技术的最先进，以及研究小鼠器官生理学的微型系统的日益可用，该物种已成为心血管生理学、病理学和发育生物学的良好模式系统。由于转基因大鼠、转基因兔和转基因猪的产生比最初预计的要困难、耗时和昂贵得多，可以预见，小鼠模型系统在未来心血管病理生理中的重要性将进一步增加。贝丝以色列女执事医疗中心的心血管部门已经做出了重大承诺，将成为专注于小鼠心脏分子生物学和生理学的领先研究中心之一。因此，我们建立了小鼠生理学核心实验室，以提供集中的服务和培训设施，确保每个参与RFA的研究人员有能力为他们的研究确定转基因和基因靶标小鼠的特征。老鼠生理学核心提供最先进的诊断方法，用于表征小鼠的心血管生理学，包括具有钙检测、血流动力学测量、心电图和心律失常监测的隔离心脏准备。拟议的核心将使这一RFA的多学科研究目标得以实现，这是传统的RO1框架无法解决的。

The goal of this Core is to provide efficient physiological characterization of transgenic mice in order to aid multi-disciplinary research projects relevant to the collaborative RO1 applications for this SCOR. The advent of gene-targeting and transgenic-techniques allowing for tissue-specific manipulations has rapidly shifted the focus of basic cardiovascular research from studying cellular and subcellular systems to the whole organ. With genetic knowledge and technologies being most advanced in the mouse, and miniaturized systems to study murine organ physiology becoming increasingly available, this species has emerged as the favorable model system for cardiovascular physiology, pathology, and developmental biology. Because the generation of transgenic rats, rabbits and pigs has turned out to be much more difficult, time consuming, and expensive than initially expected, it can be predicted that the importance of mouse model systems in cardiovascular pathophysiology will further increase in the future. The Cardiovascular Division of Beth Israel Deaconess Medical Center has made a major commitment to become one of the leading research centers focusing on the murine cardiac molecular biology and physiology. Accordingly, we have established the Mouse Physiology Core Laboratory to provide centralized service and training facilities that will ensure the ability of each participating investigator of the RFA to characterize transgenic and gene-targeted mice for their research. The Mouse Physiology Core provides state-of-the-art diagnostics for characterizing murine cardiovascular physiology, including isolated heart preparations with Ca2+ detection, hemodynamics measurements, electrocardiograms and arrhythmia monitoring. The proposed Core will enable accomplishment of multi-disciplinary research goals of this RFA that cannot be addressed by a traditional RO1 framework.