CONFORMATIONAL VARIATIONS DUE TO CATION OR LIGAND BINDING IN INTEGRINS

由于整合素中的阳离子或配体结合引起的构象变化

• 批准号: 6469047
• 负责人: INGRID STUIVER
• 金额: $ 10.66万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6469047
• 关键词: aging; biological products; biomedical resource; cognition; growth /development; human tissue; microscopy; nervous system; nucleic acids; technology /technique

We have found that the fragile X message is translated at synapses under synaptic control. We believe that this process may play a role in activity dependent synapse stabilization-maturation. To further assess function of the protein, we need to determine its subcellular localization using immuno-EM. Future studies will involve subcellular localization of the mRNA using EM in situ hybridization. Fragile-X mental retardation is the leading genetically-inherited cause of human mental retardation. Initial immunolocalization studies were performed at the NCMIR using a combination of conventional and intermediate voltage EM. Dr. Klintsova also received training in the methodologies necessary to perform immuno EM at her home institution. We now plan to begin a project using Telemicroscopy and Teletomography using specimens sent to us from Illinois.

我们已经发现脆弱的X信息在突触处被翻译 在突触的控制下 我们认为，这一进程可能发挥作用， 在活动依赖性突触稳定-成熟中。 进一步 评估蛋白质的功能，我们需要确定其亚细胞 使用免疫EM定位。 未来的研究将涉及亚细胞 使用EM原位杂交定位mRNA。 脆性x 智力迟钝是人类遗传性疾病的主要原因。 智力迟钝。 进行了初步免疫定位研究 在NCMIR中使用常规和中间 电压EM。 Klintsova博士还接受了方法学方面的培训 需要在她的家庭机构进行免疫EM。 我们现在计划 开始一个使用远程显微镜和远程断层扫描的项目， 从伊利诺伊州寄来的标本