CONFORMATIONAL VARIATIONS DUE TO CATION OR LIGAND BINDING IN INTEGRINS

我们发现脆性X信息在突触中被翻译 在突触控制下。我们认为，这一进程可能会发挥作用 在活性依赖的突触稳定-成熟中。为了进一步 要评估蛋白质的功能，我们需要确定它的亚细胞 使用免疫-EM定位。未来的研究将涉及亚细胞 应用EM原位杂交技术对该基因进行定位。脆性-X 智力低下是人类最主要的遗传原因 精神发育迟滞。进行了初步的免疫定位研究 在NCMIR使用常规和中间体的组合 电压EM。Klintsova博士还接受了方法学方面的培训 在她家的机构做免疫电镜术是必要的。我们现在计划 使用远程显微镜和电子显微照相术开始项目 从伊利诺伊州寄来的标本。

We have found that the fragile X message is translated at synapses under synaptic control. We believe that this process may play a role in activity dependent synapse stabilization-maturation. To further assess function of the protein, we need to determine its subcellular localization using immuno-EM. Future studies will involve subcellular localization of the mRNA using EM in situ hybridization. Fragile-X mental retardation is the leading genetically-inherited cause of human mental retardation. Initial immunolocalization studies were performed at the NCMIR using a combination of conventional and intermediate voltage EM. Dr. Klintsova also received training in the methodologies necessary to perform immuno EM at her home institution. We now plan to begin a project using Telemicroscopy and Teletomography using specimens sent to us from Illinois.