Endocrine control of vitellogenesis in Drosophila

果蝇卵黄发生的内分泌控制

• 批准号: 6412485
• 负责人: DAVID S RICHARD
• 金额: $ 12.81万
• 依托单位: SUSQUEHANNA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6412485
• 关键词: Drosophilidae; chemical binding; dialysis; diapause; ecdysone; egg /ovum; enzyme linked immunosorbent assay; female; hormone binding protein; hormone regulation /control mechanism; juvenile hormones; microarray technology; oogenesis; protein biosynthesis; radioimmunoassay; reproduction; steroid hormone biosynthesis; steroid hormone metabolism; vitellogenins

DESCRIPTION: (provided by the applicant): This proposal continues the re-evaluation of the roles of ecdysterolds and juvenile hormones (JH) in vitellogenesis in female Drosophila melanogaster Initiated during the original granting period (NIH GM/0D54905). Our recent work has demonstrated that JHs may be involved in the regulation of early yolk protein (YP) synthesis by follicle cells of the developing egg chamber. However, in apterous mutant, and in diapausing females, vitellogenesis can occur in the absence of JHs, but never in the absence of ecdysterolds. These observations support the model for the endocrine regulation of YP synthesis and uptake (Richard et al., 1998, 2001a). This proposal studies the levels of production and action of ecdysteroids, juvenile hormones, sex peptide and yolk proteins (YPs) by wild-type Canton-S females, Canton-S females in diapause, apterous and apterous, mutants. A successful goal of the previous granting period was to examine the processes of receptor mediated endocytosis in the trafficking of early YPs from the follicle cells, and late YPs from the hemolymph into the developing oocytes (Richard et al., 2001b). We now propose to examine further the mechanisms by which the production of the proteins alpha-adaptin, clathrin and the YP-receptor may be controlled. A cell contact regulatory mechanism between the oocyte and the nurse cells, or between the border follicle cells and both oocyte and nurse cells, is postulated and will be examined initially by immunocytochemical means. Expressed sequence tag micro-array analysis of gene expression patterns in ovaries following treatment with JHs and ecdysterolds is proposed as a simple initial step towards the incorporation of micro-array technology into our laboratory. The significance of these experiments lies with the unique position that D. melanogaster occupies in biological research as both a model insect and a model dipteran. No other insect is as well characterized In terms of its genetic make-up and while It is small, It has proved possible to surgically dissect and manipulate the organs Involved In the endocrine system. Given the Importance of some cyclorrhaphous dipteran species as vectors of human disease, it is of obvious importance that we understand the role of the endocrine system in the regulation of reproductive development.

描述：（由申请人提供）：本提案延续了 蜕皮甾酮和保幼激素在抗衰老中作用的再评价 雌性果蝇卵黄发生起始于 授权期（NIH GM/0 D54905）。我们最近的研究表明，JH可能 参与卵泡早期卵黄蛋白（YP）合成的调节 发育中的卵室细胞。然而，在无翅突变体中， 滞育的女性，卵黄发生可以发生在没有JH，但从来没有 在没有蜕皮甾体的情况下。这些观察结果支持了 YP合成和摄取的内分泌调节（Richard等人，1998年，2001年a）。 该建议研究蜕皮类固醇的产生和作用水平， 保幼激素、性肽和卵黄蛋白（YPs） 雌性、广州-S型滞育雌性、无翅和无翅型、突变体。一 上一个赠款期的成功目标是审查 受体介导的内吞作用在卵泡早期YPs运输中的作用 细胞和晚期YPs从血淋巴进入发育中的卵母细胞（Richard et 例如，2001年b）。我们现在建议进一步研究 蛋白质α-适应蛋白、网格蛋白和YP-受体的产生可以是 控制。卵母细胞和卵母细胞之间的细胞接触调节机制 滋养细胞之间或卵泡边缘细胞与卵母细胞和滋养细胞之间 细胞，假设，并将首先通过免疫细胞化学 手段基因表达谱的表达序列标签微阵列分析 在卵巢治疗后，JH和蜕皮甾体被认为是一种 将微阵列技术结合到 我们的实验室这些实验的意义在于 位置D。黑腹动物在生物学研究中既是一种模型， 昆虫和双翅目模式昆虫。没有其他昆虫能像它一样 它的基因组成，虽然它很小，但它已经被证明是可能的， 外科手术解剖和操纵内分泌系统的器官。 鉴于一些圆缝双翅目物种作为传播媒介的重要性， 人类疾病，这是显而易见的重要性，我们了解的作用， 内分泌系统对生殖发育的调节作用。