GENE EXPRESSION-ENVIRONMENT LINK IN CEREBRAL ISCHEMIA

描述：（由申请人提供）脑缺血（脑缺血急剧减少） 血液流向大脑）是创伤性脑损伤中常见的情况， 中风和心脏骤停。据报道，一些基因被激活 在脑缺血中，这些基因在影响 细胞存活和细胞死亡的过程。但目前尚不清楚如何 一个过程可以比另一个过程得到提升。因此，拟议的目标 研究目的是确定环境刺激是否可以调节基因 在脑缺血中观察到的表达并促进细胞存活 改善侮辱的认知和感觉运动效应。这项研究 将检查是否； 1）环境刺激可以调节激活的基因 脑缺血并可能促进细胞存活，2）环境 刺激可以影响基因加工成蛋白质（蛋白质携带 调节行为的信息），3）基因中存在性别差异， 脑缺血后的蛋白质表达，以及4）细胞存活 环境刺激伴随着认知和认知能力的改善 感觉运动功能。成年雄性和雌性 Wistar 大鼠将接受 缺血或假（对照）手术。然后动物将被分配到任一 4个条件：复杂环境、运动学习、锻炼和社交 条件组（对照组）脑缺血恢复后2天。老鼠 将被安置在指定的环境中 10 天。分子， 研究中将使用组织学和行为组来优化 实验设计。动物在受到环境刺激后， 分子组将被安乐死，大脑将进行基因分析 组织学组中的动物将被安乐死，并且 将分析大脑的蛋白质表达。基因表达将被确定 通过原位杂交，同时免疫细胞化学和光学解剖仪 方法将用于评估蛋白质表达。动物在行为上 安乐死前，该组将接受认知和感觉运动功能测试。 将使用绳索攀爬和旋转骑行来测试感觉运动功能 测试，同时将使用水迷宫测试认知功能。来自一个 从护理角度来看，这项研究的结果具有重要的临床意义，因为 记忆丧失是最常见且持久的认知障碍 脑缺血，常常阻碍患者的进展 康复。此外，缺血后出现的感觉运动缺陷 损害可能会影响这些幸存者的长期结果。

DESCRIPTION: (provided by applicant) Cerebral ischemia (drastic reduction in blood flow to the brain) is common condition seen in traumatic brain injury, stroke, and cardiac arrest. It has been reported that some genes are activated in cerebral ischemia and that these genes play a role in influencing the processes of both cell survival and cell death. However, it is not clear how one process can be promoted over the other. Thus, the objective of the proposed study is to determine whether environmental stimulation can modulate the gene expression seen in cerebral ischemia and promote cell survival accompanied by amelioration in the cognitive and sensory motor effects of insult. This study will examine if; 1) environmental stimulation can modulate the genes activated in cerebral ischemia and possibly promote cell survival, 2) environmental stimulation can influence the processing of genes into proteins (proteins carry the message that regulates behavior), 3) gender differences exist in gene and protein expression after cerebral ischemia, and 4) cell survival following environmental stimulation is accompanied by improvement in cognitive and sensory motor functions. Adult male and female Wistar rats will receive either ischemia or sham (control) surgery. Then animals will be assigned to either one of 4 conditions: complex environment, motor learning, exercise, and social condition (control) group 2 days after recovery from cerebral ischemia. Rats will be housed in their assigned environment for 10 days. Molecular, histological, and behavior groups will be used in the study to optimize the experimental design. After environmental stimulation, the animals in the molecular group with be euthanized and the brains will be analyzed of gene expression while animals in the histological group will be euthanized and the brains will analyzed for protein expression. Gene expression will be determined by in site hybridization while immunocytochemistry and the optical dissector method will be used to assess protein expression. Animals in the behavioral group will be tested in cognitive and sensory motor function before euthanasia. Sensory motor function will be tested using rope climbing and rotating rode tests while cognitive function will be tested using the water maze. From a nursing perspective, results from this study are important clinically because memory loss is the most frequent and enduring cognitive disability resulting from cerebral ischemia and often hinders the patient's progress during rehabilitation. Additionally, the sensory motor deficit seen following ischemic damage can affect the long-term outcome of these survivors.