FLOW CYTOMETRIC ANALYSIS OF BENIGN & MALIGNANT TUMORS
良性的流式细胞术分析
基本信息
- 批准号:6435304
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:
- 资助国家:美国
- 起止时间:至
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
Flow Cytometric immunophenotyping is a sensitive technique for analysis of benign and malignant tumors. We are studying the refinement of this technique and its application to diagnosis and measurement of prognostic markers in different systems. We are studying the flow cytometric immunophenotype of CLL and correlating the expression of specific antigens with morphology, cytogenetics and clinical course. Data from this study may provide prognostic markers for this disease. We have refined methods for flow cytometric monitoring of patient specimens for apoptosis induced by chemotherapy and have been able to detect apoptosis in specific cell populations. This has allowed us to demonstrate different sensitivities of specific cell lineages to chemotherapeutic agents. We have initiated a study of clonal cytotoxic T-cell populations that arise in patients with B-cell neoplasia to determine prognostic importance as well as resulting difficulties in minimal residual disease detection. As a result, we have found that clonal cytotoxic T-cell proliferations occur with surprising frequency in hairy cell leukemia. The laboratory has an ongoing interest in detection of minimal and residual lymphoma. We are using multiparametric approaches to improve the sensitivity of detection of monoclonal B-cell populations. By targeting abnormal patterns of antigen expression (eg CD10, CD5, CD23, FMC7, or abnormal intensity of antigen expression) by neoplastic B-cells in light chain detection, we are attempting to detect very small numbers of neoplastic B-cells among admixed polyclonal B-cells. The laboratory is studying specific Flow Cytometric markers of various lymphoma sub-groups to improve diagnostic accuracy. The Flow Cytometry Laboratory is developing methods for Flow Cytometric analysis to detect EBV gene expression in the research and clinical setting.
流式细胞术免疫表型分析是一种敏感的技术,用于分析良性和恶性肿瘤。我们正在研究这种技术的改进及其在不同系统中诊断和测量预后标志物的应用。我们正在研究CLL的流式细胞术免疫表型,并将特异性抗原的表达与形态学、细胞遗传学和临床病程相关联。这项研究的数据可能提供这种疾病的预后标志物。我们已经改进了流式细胞术监测化疗诱导的细胞凋亡患者标本的方法,并已能够检测特定细胞群的细胞凋亡。这使我们能够证明特定细胞系对化疗药物的不同敏感性。我们已经启动了一项研究,克隆细胞毒性T细胞群体中出现的B细胞瘤形成的患者,以确定预后的重要性,以及在微小残留疾病检测的困难。结果,我们发现克隆性细胞毒性T细胞增殖在毛细胞白血病中以惊人的频率发生。该实验室一直对微小和残留淋巴瘤的检测感兴趣。我们正在使用多参数方法来提高单克隆B细胞群体检测的灵敏度。通过在轻链检测中靶向肿瘤性B细胞的异常抗原表达模式(例如CD10、CD5、CD23、FMC7或异常抗原表达强度),我们试图在混合的多克隆B细胞中检测非常少量的肿瘤性B细胞。该实验室正在研究各种淋巴瘤亚组的特异性流式细胞术标记物,以提高诊断准确性。流式细胞术实验室正在开发流式细胞术分析方法,以检测研究和临床环境中的EBV基因表达。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Maryalice Stetler-Stevenson其他文献
Maryalice Stetler-Stevenson的其他文献
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{{ truncateString('Maryalice Stetler-Stevenson', 18)}}的其他基金
Flow Cytometric Analysis of Benign and Malignant Tumors
良性和恶性肿瘤的流式细胞术分析
- 批准号:
7331306 - 财政年份:
- 资助金额:
-- - 项目类别:
FLow Cytometric Detection of Malignant Cells in Body Fluids
体液中恶性细胞的流式细胞仪检测
- 批准号:
8350143 - 财政年份:
- 资助金额:
-- - 项目类别:
Flow Cytometric Analysis of Benign and Malignant Tumors
良性和恶性肿瘤的流式细胞术分析
- 批准号:
7068862 - 财政年份:
- 资助金额:
-- - 项目类别:
Flow Cytometric Analysis of Benign and Malignant Tumors
良性和恶性肿瘤的流式细胞术分析
- 批准号:
9154363 - 财政年份:
- 资助金额:
-- - 项目类别:
TIMP-1 EXPRESSION BY NORMAL LYMPHOCYTES & IN LYMPHOID NE
正常淋巴细胞表达 TIMP-1
- 批准号:
6435306 - 财政年份:
- 资助金额:
-- - 项目类别:
Flow Cytometric Detection of Malignant Cells in Body Fluids
体液中恶性细胞的流式细胞术检测
- 批准号:
10262750 - 财政年份:
- 资助金额:
-- - 项目类别:
Flow Cytometric Analysis of Benign and Malignant Tumors
良性和恶性肿瘤的流式细胞术分析
- 批准号:
10262791 - 财政年份:
- 资助金额:
-- - 项目类别:
Flow Cytometric Analysis of Benign and Malignant Tumors
良性和恶性肿瘤的流式细胞术分析
- 批准号:
7735388 - 财政年份:
- 资助金额:
-- - 项目类别:
Flow Cytometric Detection of Malignant Cells in Body Fluids
体液中恶性细胞的流式细胞术检测
- 批准号:
9344169 - 财政年份:
- 资助金额:
-- - 项目类别:
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