FLOW CYTOMETRIC ANALYSIS OF BENIGN & MALIGNANT TUMORS

流式细胞免疫分型技术是分析良、恶性肿瘤的一种灵敏技术。我们正在研究这项技术的改进及其在不同系统中诊断和测量预后标记物的应用。我们正在研究CLL的流式细胞仪免疫表型，以及特异性抗原的表达与细胞形态、细胞遗传学和临床病程的相关性。这项研究的数据可能会为这种疾病提供预后标志。我们有改进的方法来监测患者标本中化疗诱导的细胞凋亡，并能够检测特定细胞群中的细胞凋亡。这使得我们能够展示特定细胞系对化疗药物的不同敏感性。我们已经开始了对B细胞肿瘤患者中出现的克隆性细胞毒性T细胞群的研究，以确定预后的重要性以及由此导致的微小残留病检测的困难。因此，我们发现，在毛细胞白血病中，克隆性细胞毒性T细胞增殖的频率令人惊讶。实验室一直对检测微小和残留的淋巴瘤感兴趣。我们正在使用多参数方法来提高检测单克隆性B细胞群体的灵敏度。通过在轻链检测中针对肿瘤B细胞抗原表达的异常模式(如CD10、CD5、CD23、FMC7或抗原表达异常强度)，我们试图在混合的多克隆B细胞中检测到极少量的肿瘤B细胞。该实验室正在研究不同淋巴瘤亚组的特定流式细胞术标记物，以提高诊断的准确性。流式细胞术实验室正在开发流式细胞术分析方法，以在研究和临床环境中检测EBV基因的表达。

Flow Cytometric immunophenotyping is a sensitive technique for analysis of benign and malignant tumors. We are studying the refinement of this technique and its application to diagnosis and measurement of prognostic markers in different systems. We are studying the flow cytometric immunophenotype of CLL and correlating the expression of specific antigens with morphology, cytogenetics and clinical course. Data from this study may provide prognostic markers for this disease. We have refined methods for flow cytometric monitoring of patient specimens for apoptosis induced by chemotherapy and have been able to detect apoptosis in specific cell populations. This has allowed us to demonstrate different sensitivities of specific cell lineages to chemotherapeutic agents. We have initiated a study of clonal cytotoxic T-cell populations that arise in patients with B-cell neoplasia to determine prognostic importance as well as resulting difficulties in minimal residual disease detection. As a result, we have found that clonal cytotoxic T-cell proliferations occur with surprising frequency in hairy cell leukemia. The laboratory has an ongoing interest in detection of minimal and residual lymphoma. We are using multiparametric approaches to improve the sensitivity of detection of monoclonal B-cell populations. By targeting abnormal patterns of antigen expression (eg CD10, CD5, CD23, FMC7, or abnormal intensity of antigen expression) by neoplastic B-cells in light chain detection, we are attempting to detect very small numbers of neoplastic B-cells among admixed polyclonal B-cells. The laboratory is studying specific Flow Cytometric markers of various lymphoma sub-groups to improve diagnostic accuracy. The Flow Cytometry Laboratory is developing methods for Flow Cytometric analysis to detect EBV gene expression in the research and clinical setting.