PROBING ELASTICITY WITH THE ATOMIC FORCE MICROSCOPE

用原子力显微镜探测弹性

• 批准号: 6413460
• 负责人: EMILIOS K DIMITRIADIS
• 金额: --
• 依托单位: OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6413460
• 关键词: atomic force microscopy; bioimaging /biomedical imaging; elasticity; gel; method development; nanotechnology

The atomic force microscope (AFM) is increasingly becoming a first line tool for measuring material properties at microscopic scales. When it comes to soft biological materials such as cells, however, serious problems have been identified with both the widely used measuring approach and with the data analysis methods. Here we develop an improved experimental approach and develop the theoretical framework for the analysis of collected AFM force-displacement data. We also establish the applicability domain for both the experimental and theoretical frameworks. The results of the project are being verified by extracting elastic moduli for soft gels using the AFM and then comparing those results with those obtained from macroscopic measurements.

原子力显微镜(AFM)正日益成为在微观尺度上测量材料性质的第一线工具。然而，当涉及到细胞等软生物材料时，广泛使用的测量方法和数据分析方法都存在严重问题。在这里，我们发展了一种改进的实验方法，并建立了分析收集到的AFM的理论框架 力-位移数据。我们还建立了实验框架和理论框架的适用范围。该项目的结果是通过使用原子力显微镜提取软凝胶的弹性模数，然后将这些结果与宏观测量结果进行比较来验证的。