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Atomic Force Microscopy Imaging of Protein/DNA Complexes

Atomic Force Microscopy Imaging of Protein/DNA Complexes
蛋白质/DNA 复合物的原子力显微镜成像
批准号:
7013049
负责人:
EMILIOS K DIMITRIADIS
金额:
$0.0万
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至

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中文摘要
翻译
HU是一种组蛋白样蛋白,在哺乳动物和细菌细胞中均有表达。在细菌细胞中,除了基因调控外,它似乎在DNA构象中有一个不太清楚的作用。在这里,我们使用原子力显微镜研究了野生型和突变型胡与长,线性和质粒DNA分子的相互作用。在新切割的云母表面上获得分子及其复合物的图像。然后对这些图像进行定性和定量分析,旨在阐明DNA压实的机制。在新鲜切割的云母上混合和孵育后,在空气中进行成像。
英文摘要
HU is a histone-like protein expressed in both mammalian and bacterial cells. In bacterial cells it seems to have a not well-understood role in DNA conformation in addition to gene regulation. Here, we examine the interaction of wild-type and mutant forms of HU with long, linear and plasmid DNA molecules using the atomic force microscope. Images are obtained of molecules and their complexes on freshly cleaved mica surfaces. These images are then analyzed qualitatively and quantitatively aiming at elucidating the mechanics of DNA compaction. Imaging is performed in air after mixing and incubating on freshly cleaved mica.
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Magnetic Resonance Elastography
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