Atomic Force Microscopy Imaging of Protein/DNA Complexes

蛋白质/DNA 复合物的原子力显微镜成像

• 批准号: 7013049
• 负责人: EMILIOS K DIMITRIADIS
• 金额: --
• 依托单位: OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7013049
• 关键词: DNA; atomic force microscopy; conformation; genetic regulation; imaging /visualization /scanning; nanotechnology; plasmids; proteins

HU is a histone-like protein expressed in both mammalian and bacterial cells. In bacterial cells it seems to have a not well-understood role in DNA conformation in addition to gene regulation. Here, we examine the interaction of wild-type and mutant forms of HU with long, linear and plasmid DNA molecules using the atomic force microscope. Images are obtained of molecules and their complexes on freshly cleaved mica surfaces. These images are then analyzed qualitatively and quantitatively aiming at elucidating the mechanics of DNA compaction. Imaging is performed in air after mixing and incubating on freshly cleaved mica.

HU是在哺乳动物和细菌细胞中表达的组蛋白样蛋白。在细菌细胞中，除了基因调控外，它似乎在DNA构象中具有不太清楚的作用。在这里，我们研究的野生型和突变形式的HU与长，线性和质粒DNA分子的相互作用，使用原子力显微镜。图像获得的分子和它们的复合物在新切割的云母表面。然后对这些图像进行定性和定量分析，旨在阐明DNA压缩的机制。在新鲜切割的云母上混合和孵育后，在空气中进行成像。