PROMOTER- BASED ESTIMATION ANALYSIS
PROMOTER- BASED ESTIMATION ANALYSIS
批准号:
6459455
负责人:
Hiroki Yokota
金额:
$11.18万
依托单位国家:
美国
项目类别:
财政年份:
2002
资助国家:
美国
项目状态:
已结题
起止时间:
2002-05-10 至 2004-04-30
中文摘要
描述(由申请人提供):长期目标是发展一个
英文摘要
DESCRIPTION (provided by applicant): The long-term objective is to develop an
integrated computational, biochemical approach in gene regulation, where a
computational part incorporates a large volume of gene expression data with
genomic information and a biochemical part will test experimentally the
predictions made in the computational part. The specific goal is to develop
such an approach by integrating a promoter-based estimation analysis with a
promoter competition assay. The promoter-based estimation analysis was
developed by the Principal Investigator (PI) to model a pattern of mRNA
expression from promoter DNA sequences. The promoter competition assay was
developed in the PI's laboratory to test a role of particular cis-acting
regulatory elements in gene expression. In the proposal, functional
significance of a group of cis-acting elements will be predicted for a model
system in the promoter-based estimation analysis and the prediction will be
tested in the promoter competition assay.
As a model system the gene expression of a family of human matrix metal
loproteinases will be used, and mechanical shear will be applied as
environmental stress. The specific aims of the project are: (1) to model the
mRNA expression of MMPs by using a least-square modeler in the promoter-based
estimation analysis, and (2) to test predicted functional role of particular
cis-acting elements in shear responses by using the promoter competition
assay. In modeling the expression of MMP mRNA, the mRNA level will be
determined by using real-time reverse-transcription/polymerase chain reaction.
In the promoter-based estimation analysis, the observed mRNA expression will
be modeled by a frequency of cis-acting regulatory elements on promoter DNA
sequences. A standard linear algebra will be used, and the engenvalue
analysis will be conducted to predict the functional role of specific
cis-acting elements in shear responses. The predicted role of cis-acting
elements will be tested in the promoter competition assay, where exogenous DNA
fragments consisting of specific cis-acting elements will be transiently
transferred to the cells.
The integrated computational, biochemical assay system developed in this
proposal will be used to interpret the expression pattern of many genes under
genetic or environmental stress and contribute to elucidating molecular
mechanisms in complex cellular stress responses.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Effect of skeletal compression on tumor growth and migration
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批准号:9889087
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项目类别:
-
资助金额:$7.88万
-
财政年份:2019
-
负责人:Hiroki Yokota
-
依托单位:
Mechanical response of osteoblasts in 3D matrix
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批准号:6773674
-
项目类别:
-
资助金额:$22.82万
-
财政年份:2004
-
负责人:Hiroki Yokota
-
依托单位:
Mechanical response of osteoblasts in 3D matrix
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批准号:6883254
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项目类别:
-
资助金额:$20.54万
-
财政年份:2004
-
负责人:Hiroki Yokota
-
依托单位:
Mechanical response of osteoblasts in 3D matrix
-
批准号:7046778
-
项目类别:
-
资助金额:$20.06万
-
财政年份:2004
-
负责人:Hiroki Yokota
-
依托单位:
BONE AND KNEE LOADING
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批准号:6937255
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项目类别:
-
资助金额:$7.58万
-
财政年份:2004
-
负责人:Hiroki Yokota
-
依托单位:
Mechanical response of osteoblasts in 3D matrix
-
批准号:7215630
-
项目类别:
-
资助金额:$19.48万
-
财政年份:2004
-
负责人:Hiroki Yokota
-
依托单位:
BONE AND KNEE LOADING
-
批准号:6828455
-
项目类别:
-
资助金额:$7.58万
-
财政年份:2004
-
负责人:Hiroki Yokota
-
依托单位:
Mechanical Loading and Bone
-
批准号:8261663
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项目类别:
-
资助金额:$33.26万
-
财政年份:2002
-
负责人:Hiroki Yokota
-
依托单位:
MECHANICAL LOADING AND BONE
-
批准号:7742667
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项目类别:
-
资助金额:$21.98万
-
财政年份:2002
-
负责人:Hiroki Yokota
-
依托单位:
Mechanical Loading and Bone
-
批准号:8312379
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项目类别:
-
资助金额:$33.26万
-
财政年份:2002
-
负责人:Hiroki Yokota
-
依托单位:
Mechanical Loading and Bone
-
批准号:8196771
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项目类别:
-
资助金额:$29.16万
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财政年份:2002
-
负责人:Hiroki Yokota
-
依托单位:
Mechanical Loading and Matrix Metalloproteinase
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批准号:6661332
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项目类别:
-
资助金额:$18.81万
-
财政年份:2002
-
负责人:Hiroki Yokota
-
依托单位:
Mechanical Loading and Bone
-
批准号:8516459
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项目类别:
-
资助金额:$31.6万
-
财政年份:2002
-
负责人:Hiroki Yokota
-
依托单位:
MECHANICAL LOADING AND BONE
-
批准号:7184451
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项目类别:
-
资助金额:$28.83万
-
财政年份:2002
-
负责人:Hiroki Yokota
-
依托单位:
MECHANICAL LOADING AND BONE
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批准号:7567572
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项目类别:
-
资助金额:$22.2万
-
财政年份:2002
-
负责人:Hiroki Yokota
-
依托单位:
Mechanical Loading and Matrix Metalloproteinase
-
批准号:6541174
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项目类别:
-
资助金额:$21.46万
-
财政年份:2002
-
负责人:Hiroki Yokota
-
依托单位:
Mechanical Loading and Bone
-
批准号:8759068
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项目类别:
-
资助金额:$34.32万
-
财政年份:2002
-
负责人:Hiroki Yokota
-
依托单位:
Mechanical Loading and Bone
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批准号:9099729
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项目类别:
-
资助金额:$34.32万
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财政年份:2002
-
负责人:Hiroki Yokota
-
依托单位:
MECHANICAL LOADING AND BONE
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批准号:7043055
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项目类别:
-
资助金额:$30.82万
-
财政年份:2002
-
负责人:Hiroki Yokota
-
依托单位:
MECHANICAL LOADING AND BONE
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批准号:7345477
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项目类别:
-
资助金额:$22.2万
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财政年份:2002
-
负责人:Hiroki Yokota
-
依托单位:
海外基金