权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

BONE AND KNEE LOADING

骨骼和膝盖负载

基本信息

批准号：
6937255
负责人：
Hiroki Yokota
金额：
$ 7.58万
依托单位：
INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-09-01 至 2007-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6937255
关键词：
aging biological models biomechanics bone development computed axial tomography femur femur fracture histology knee laboratory mouse mechanical pressure mechanical stress morphometry skeletal stress

项目摘要

DESCRIPTION (provided by applicant): One of the major health needs in the elderly is to strengthen bone and prevent bone mass. In this project, we propose to investigate a novel mechanical loading model- knee joint loading - to stimulate bone formation and to strengthen mechanical properties of femur and a femoral neck. In our preliminary studies using mouse ulnae, bone formation was stimulated by loading an elbow joint. Furthermore, the loads required to induce bone formation with this elbow-loading model were smaller than that with a traditional longitudinal ulna-loading model. These results suggest that femur and a femoral neck can be strengthened by mechanical loads that are applied to a knee joint. The specific goal of the proposed project is to evaluate the effects of loads applied to the knee joint on bone mass and mechanical strength of femur and a femoral neck. The hypothesis, tested in the proposed study, is: "Low-amplitude sinusoidal mechanical loads with < 1 N at -20 Hz on a knee joint will stimulate new bone formation in femur and a femoral neck, and increase resistance to fracture." In the proposed study we will first identify the appropriate knee-loading conditions by examining streaming potentials and dissipation energy. Mechanical loads at physiological frequencies will be applied to the knee joint and streaming potentials and dissipation energy will be determined in the femur. Using the conditions that induce streaming potentials and elevate dissipation energy, we will then evaluate the loading effects through bone histomorphometry, micro-Computed Tomography (mu CT), and biomechanical tests. C57BL/6 mice will be given mechanical loads for 3 min per day for 3 consecutive days. After staining newly formed bone with calcein, bone morphometric parameters will be determined along a femoral midshaft. Three-dimensional morphology will be determined with mu CT, and mechanical parameters such as ultimate force, and work to failure will be determined. The proposed study will contribute to development of a novel loading model to strengthen bone without causing deformation of fragile bone in the elderly.

说明(申请人提供)：老年人的主要健康需求之一是强骨和预防骨量。在这个项目中，我们建议研究一种新的机械加载模型-膝关节加载-以刺激骨形成并增强股骨和股骨颈的力学性能。在我们使用小鼠尺骨进行的初步研究中，通过加载肘关节来刺激骨形成。此外，与传统的尺骨纵向加载模型相比，该肘部加载模型诱导骨形成所需的载荷更小。这些结果表明，股骨和股骨颈可以通过施加在膝关节上的机械载荷来加强。该项目的具体目标是评估施加在膝关节上的载荷对股骨和股骨颈的骨量和机械强度的影响。这项研究中提出的假设是：“在-20赫兹时，在膝关节上施加-lt；1N的低幅度正弦机械载荷将刺激股骨和股骨颈新骨的形成，并增加对骨折的抵抗力。”在拟议的研究中，我们将首先通过检查流动电位和耗散能量来确定合适的膝关节负荷条件。生理频率的机械载荷将施加在膝关节上，股骨中将确定流动电位和耗散能量。利用诱导流动电位和提高耗散能量的条件，我们将通过骨组织形态计量学、微型计算机断层扫描(MU CT)和生物力学测试来评估负荷效应。C57BL/6小鼠接受机械负荷，每天3min，连续3d。在用钙黄素对新形成的骨进行染色后，将沿着股骨中段测定骨形态计量学参数。用MU CT确定三维形态，并确定极限受力、从工作到失效等力学参数。这项研究将有助于开发一种新的负荷模式，在不引起老年人脆弱骨变形的情况下加强骨骼。