Cancer Center Tumor Phenotyping Core

描述(由申请人提供)： 1996年，俄亥俄州的所有癌症死亡率在50个州中排名第13位 各州和哥伦比亚特区。俄亥俄州的死亡率排名第16位 肺癌，第12位结直肠癌，第6位乳腺癌 死亡。辛辛那提及其周边地区的面积在 黑人、白人、男性和女性的癌症死亡率。汉密尔顿县 (辛辛那提大学网站)在统计上显著高于 癌症发病率比俄亥俄州的总体发病率还要高。这些数据激起了临床医生的热情， 研究人员和管理员共同开发协作计划， 激发对癌症基础生物学的新研究，并促进项目 以便将这一新知识转化为临床护理。这项建议 是一系列讨论小组直接产生的四个结果之一， 包括临床医生和基础研究人员，制定计划战略，以 满足这些临床需求。临床/翻译和基础研究人员 我觉得组织研究的机构资源匮乏 而一个肿瘤表型核心将大大加强这项研究 在医学院的工作能力。该核心将与以下两个核心密切合作 其他建议的核心，提供(1)基因组学/蛋白质组学和(2) 常规组织学、组织微阵列的制备和激光捕获 显微解剖。肿瘤表型核心将为 自动免疫组织化学、原位杂交、原位荧光 杂交(FISH)、RNA和DNA分离、荧光显微镜和REAL 时间聚合酶链式反应。这一核心将为NCI目前资助的30个国家提供资源 该机构的研究人员，以及提供种子赠款资金 启动项目，鼓励更多与癌症相关的研究 机构。将根据对提案的审查来使用资源 内部咨询委员会，将提供人员以及50%的 已批准项目的供应成本。这个核心是洋流的扩展 病理学系中为临床和研究服务的设施 机构中的需要。人员增加和供应增加 美元将使这个核心为无数癌症研究人员提供服务 社区。

DESCRIPTION (provided by applicant): In 1996, Ohio's death rate from all cancers was 13th highest among the 50 states and the District of Columbia. Ohio ranked 16th highest for death rates from lung cancer, 12th from colorectal cancer and 6th for breast cancer deaths. The area in and around Cincinnati is in the top 10th percentile of mortality from cancer for Blacks, Whites, males and females. Hamilton County (site of the University of Cincinnati) has a statistically significant higher cancer incidence than Ohio overall. These data have ignited clinicians, researchers, and administrators to develop collaborative programs that will spark new investigations into the basic biology of cancer and foster programs to allow translation of this new knowledge into clinical care. This proposal is one of four that are the direct result of a series of discussion groups, including clinicians and basic researchers, to develop program strategies to meet these clinical needs. Both clinical/translational and basic researchers felt there was a paucity of institutional resources for tissue-based research and that a Tumor Phenotyping Core would significantly enhance the research capacity in the College of Medicine. This core will work closely with two of the other proposed cores, which provide (1) genomics/proteomics and (2) routine histology, preparation of tissue microarrays, and laser capture microdissection. The Tumor Phenotyping Core will provide resources for automated immunohistochemistry, in situ hybridization, fluorescence in situ hybridization (FISH), RNA and DNA isolation, fluorescence microscopy, and real time PCR. This core will provide resources to the 30 currently NCI funded investigators in the institution, as well as providing seed grant monies for start up projects to encourage additional cancer related research in the institution. Resources will be utilized based upon review of proposals by an Internal Advisory Committee and will provide personnel as well as 50% of supply costs for approved projects. This core is an expansion of a current facility in the Department of Pathology that serves clinical and research needs in the institution. Expansion of personnel and availability of supply dollars will allow this core to serve numerous investigators in the cancer community.