Compact Centrifugal Microfluidics Based BWA Analyzer

基于 BWA 的紧凑型离心微流控分析仪

• 批准号: 6555468
• 负责人: DALIBOR HODKO
• 金额: $ 16.22万
• 依托单位: NANOGEN, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-03 至 2003-09-01
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6555468
• 关键词: analytical method; biomedical automation; biomedical equipment development; bioterrorism /chemical warfare; centrifugation; electrochemistry; electrophoresis; fluid flow; fluorescent dye /probe; microarray technology; microorganism genetics; portable biomedical equipment

DESCRIPTION (provided by applicant): Rapid detection of biological warfare agents (BWA) in field is critical to provide appropriate countermeasures to deployment of weapons of mass destruction. The first responders in the field need instruments which will be portable (less than 10 kg), provide specific detection of a number of pathogens (desirable 810), with analysis time of ca 1-2 hours and which will not require special skills for their operation. There are numerous analyzers available for detection of pathogens in liquid samples, however, a major obstacle for their efficient use in the field is that they do not integrate sample preparation with the detection system. The methods based on analyzing DNA characteristic for each pathogen in the sample have been shown to provide most specific response and can be used when detection of multiple agents is needed. Analysis of nucleic acids requires a series of analytical steps which are difficult to integrate and automate in a field portable instrument. A unique microfluidic system is proposed which integrates pathogen separation from complex liquid samples, pathogen lysis, amplification of DNA and detection. Nanogen's commercially available electronic chip array for electrophoretic accumulation of DNA and fluoroprobes and for detection of DNA will be modified to include sample preparation step. A disposable DNA chip array will be developed with integrated sample preparation steps. The proposed method builds on developed technology and on incorporation of several innovations which will assure a quick development of a new portable field detector for detection of multiple biological agents and will be very flexible to accommodate other or new types of analytes.

描述（由申请人提供）：现场快速检测生物战剂（BWA）对于为大规模杀伤性武器的部署提供适当的对策至关重要。现场的急救人员需要便携式（小于 10 公斤）的仪器，能够对多种病原体（理想的 810 种）进行特异性检测，分析时间约为 1-2 小时，并且不需要特殊的操作技能。有许多分析仪可用于检测液体样品中的病原体，然而，它们在现场有效使用的一个主要障碍是它们没有将样品制备与检测系统集成。基于分析样本中每种病原体 DNA 特征的方法已被证明可以提供最特异的响应，并且可以在需要检测多种病原体时使用。核酸分析需要一系列分析步骤，这些步骤很难在现场便携式仪器中集成和自动化。提出了一种独特的微流体系统，该系统集成了从复杂液体样品中分离病原体、裂解病原体、DNA 扩增和检测。 Nanogen 用于 DNA 和荧光探针电泳积累以及 DNA 检测的市售电子芯片阵列将进行修改，以包括样品制备步骤。将开发一次性 DNA 芯片阵列，并集成样品制备步骤。所提出的方法建立在已开发的技术和多项创新的基础上，这将确保快速开发用于检测多种生物制剂的新型便携式现场检测器，并且将非常灵活地适应其他或新型分析物。