MICROFLUIDIC SYSTEM FOR THE DETECTION OF MICROORGANISMS

用于微生物检测的微流控系统

基本信息

  • 批准号:
    2865465
  • 负责人:
  • 金额:
    $ 10万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1999
  • 资助国家:
    美国
  • 起止时间:
    1999-02-15 至 1999-12-14
  • 项目状态:
    已结题

项目摘要

Miniaturization of analytical systems offers considerable potential for overcoming many of the problems associated with traditional instrumental methods of analysis in biomedical, pharmaceutical and diagnostics applications, namely the handling of small amounts of costly reagents and errors introduced by the operator in preparation of the reagents. The advantages of performing analyses on-chip comprise reduced power requirements, the ability to perform multiple analyses simultaneously in a portable instrument and enhancement of the sensitivity of the measurements as the analyte volumes measured are at nano-scale. The objective of this proposal is to develop a new type of portable microfluidic system for the detection of biological cells and microorganisms in liquid samples such as blood, plasma and/or aqueous or non-aqueous samples. The miniaturized instrument will provide a unique interface between real world samples taken usually in the range of a milliliter(s), and will concentrate them (up to 10,000 fold) into nanoliter volume within the chip microchannels. The microorganisms are detected through on-chip polymerase chain reaction (PCR) amplification of microorganism characteristic DNA. The system is based on two innovations, one for concentrating the microorganisms and the other provides a new simplified detection principle of the DNA amplicons. The principles developed in this project will provide a basis for the development of a new generation of highly sensitive portable instruments capable of detecting microorganisms at concentrations as low as 10 cells per ml. PROPOSED COMMERCIAL APPLICATIONS: The proposed microfluidic system will find a large number of applications in future microfluidic instrumentation for integration of basic components for concentration, transport and detection of biologic and inorganic particles of varying size in liquid samples. Potential commercial applications of the developed instrument will include biomedical diagnostics at the point-of-care or bedside testing, in-field environmental monitoring and on production lines in the food, chemical, pharmaceutical and biotechnology industries.
分析系统的小型化为克服生物医学、制药和诊断应用中与传统仪器分析方法相关的许多问题提供了相当大的潜力,即处理少量昂贵的试剂和操作者在制备试剂时引入的错误。在芯片上进行分析的优势包括降低了功耗需求,能够在便携式仪器中同时执行多个分析,并提高了测量的灵敏度,因为所测量的分析物体积为纳米级。这项建议的目的是开发一种新型的便携式微流控系统,用于检测血液、血浆和/或水或非水样品中的生物细胞和微生物。这种小型化的仪器将在通常在毫升(S)范围内采集的真实世界样品之间提供一个独特的接口,并将它们在芯片微通道中浓缩成纳升体积(高达10,000倍)。通过芯片上的聚合酶链式反应(PCR)扩增微生物特征DNA来检测微生物。该系统基于两个创新,一个是浓缩微生物,另一个是提供了一种新的简化的DNA扩增检测原理。本项目开发的原理将为新一代高灵敏度便携式仪器的开发奠定基础,该仪器能够检测浓度低至每毫升10个细胞的微生物。拟议的商业应用:拟议的微流控系统将在未来的微流控仪器中获得大量应用,用于集成基本组件,用于浓缩、传输和检测液体样品中不同大小的生物和无机颗粒。开发的仪器的潜在商业应用将包括在护理点或床边测试、现场环境监测以及食品、化工、制药和生物技术行业的生产线上的生物医学诊断。

项目成果

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DALIBOR HODKO其他文献

DALIBOR HODKO的其他文献

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{{ truncateString('DALIBOR HODKO', 18)}}的其他基金

Electric-Field Assisted Isothermal On-Chip Amplification of DNA
电场辅助 DNA 片上等温扩增
  • 批准号:
    7052985
  • 财政年份:
    2006
  • 资助金额:
    $ 10万
  • 项目类别:
Compact Centrifugal Microfluidics Based BWA Analyzer
基于 BWA 的紧凑型离心微流控分析仪
  • 批准号:
    6555468
  • 财政年份:
    2002
  • 资助金额:
    $ 10万
  • 项目类别:
A Centrifugal Microfluidics Based Pathogen Analyzer
基于离心微流控的病原体分析仪
  • 批准号:
    7020732
  • 财政年份:
    2002
  • 资助金额:
    $ 10万
  • 项目类别:
A Centrifugal Microfluidics Based Pathogen Analyzer
基于离心微流控的病原体分析仪
  • 批准号:
    6881749
  • 财政年份:
    2002
  • 资助金额:
    $ 10万
  • 项目类别:
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