Prototype Automated Protein Crystallization Monitoring

自动蛋白质结晶监测原型

• 批准号: 6485653
• 负责人: JOHN M ROSENBERG
• 金额: $ 18.39万
• 依托单位: XTAL MATIC, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2005-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6485653
• 关键词: X ray crystallography; biomedical automation; biomedical equipment development; computer program /software; computer system design /evaluation; crystallization; digital imaging; molecular biology information system; monitoring device; protein structure; robotics

The primary goal of this project is to build and test a prototype instrument for high throughput protein crystallography; it would automate the monitoring and storage of crystallization experiments form their initial "set up" (typically on a robot) through he final extraction for structure determination. We call this instrument a "Protein Crystallization Monitor Station" (or PCMS). It would store and monitor large number of crystallization experiment (in exchangeable cassettes of 180 microplates containint 17,280 individual experiments) at constant temperature and humidity. Crystallizations would be monitored by digital image capture; the digital image would be initially screened by machine vision software that would significantly reduce the number of images requiring human interpretation. The software associated with the PCMS would be integrated with the XtalGrow software developed in the Principal Investigator's laboratory. PROPOSED COMMERCIAL APPLICATIONS: This project will develop an automated instrument that would facilitate protein crystallization for both high-throughput and conventional protein crystallization efforts.

该项目的主要目标是构建和测试高通量蛋白质晶体学的原型仪器;它将自动监控和存储结晶实验，从最初的“设置”（通常在机器人上）到最终的结构测定提取。 我们称这种仪器为“蛋白质结晶监测站”（或PCMS）。它将在恒温恒湿条件下储存和监测大量的结晶实验（在180个微孔板的可更换盒中，包含17，280个单独的实验）。结晶过程将通过数字图像采集进行监测;数字图像将首先由机器视觉软件进行筛选，这将大大减少需要人工判读的图像数量。与PCMS相关的软件将与主要研究者实验室开发的XtalGrow软件集成。拟议的商业应用：该项目将开发一种自动化仪器，以促进蛋白质结晶的高通量和传统的蛋白质结晶的努力。