Coating for improved sensitivity and range of microarray

描述(申请人提供)：医学上的一项重要发展 能够快速、可靠地对个人进行疾病筛查和 最近的遗传标记，可能会使个体预先准备好发展 在他们一生中的疾病。以临床诊断学为基础的发展 基因表达谱因遗传而趋于成熟 人类基因组计划收集的信息，动物的产生 研究人类疾病的模型和许多其他基因组和蛋白质组方法 应用于破解过去疾病的分子致病机制 十年。这些进步的一个结果是需要增加探头密度 在微阵列表面。 STS生物聚合物正在开发一种三维表面处理 (GRAGE-COATTM)，这将允许增加遗传标记的密度 转移到微阵列上。该项目的第一阶段部分将决定 决定GRAVER-COATTM灵敏度和动态范围的变量。 微阵列表面。能够结合一系列蛋白质和基因的表面 将准备好探测器。最后，GRAVER-COATTM芯片的性能 打印了蛋白质和DNA探针的人将与使用的表面进行比较 目前。在第二阶段，我们将专注于涂料配方，提供 最高的灵敏度和最宽的动态范围，便于进一步开发和 小型化。 建议的商业应用：不可用

DESCRIPTION (provided by applicant): An important development in medicine has been the ability to quickly and reliably screen individuals for diseases and more recently genetic markers which may pre-dispose individuals to develop illnesses during their lifetime. The development of clinical diagnostics based on gene expression profiles is approaching maturation due to genetic information gathered by the Human Genome Project, the generation of animal models to study human disease and many other genomic and proteomic approaches being applied to decipher the molecular pathogenisis of disease over the last decade. One product of these advances is the need to increase probe densities on microarray surfaces. STS Biopolymers is developing a three-dimensional surface treatment (GRAFT-COATTM) that will allow an increase in the density of genetic markers onto microarrays. The Phase I portion of this project will determine the variables that determine the sensitivity and dynamic range of GRAFT-COATTM. microarray surfaces. Surfaces capable of binding a range of protein and genetic probes will be prepared. Finally, the performance of GRAFT-COATTM microarrays printed with protein and DNA probes will be compared to those of surfaces used currently. In Phase II we will focus on coating formulations providing the highest sensitivity and broadest dynamic range for further development and miniaturization. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE