TEMPERATURE JUMP INDUCED FOLDING IN MUTANTS OF GREEN FLUORESCENT PROTEINS

我们有兴趣研究绿豆的几个变种 具有特定氨基酸突变的荧光蛋白(GFP) 接近天然荧光发色团。这些突变将允许 美国将探测各种非辐射过程(电荷转移等)AS 以及它们对辐射寿命的影响。我们也是 有兴趣研究折叠动力学的细节 这些蛋白质。GFP有一个相当尖锐的熔化曲线，因此 在RLBL的仪器上提供的大温度跳跃 将很好地作为折叠动态过程的触发器， 这将通过GFP的荧光变化进行监测

We are interested in investigating several mutants of the green fluorescent protein (GFP) which have specific amino acid mutations near the natural fluorescent chromophore. These mutations will allow us to probe various non radiative processes (charge transfer, etc.) as well as their effect on the radiative lifetimes. We are also interested in investigating the details of the folding dynamics of these proteins. GFPs have a reasonably sharp melting curve and thus the large temperature jumps available on the instrument at the RLBL would serve us well as a trigger for the folding dynamic process, which would be monitored by the change in fluorescence of the GFPs