FACSCALIBER

描述(由申请人提供)：微生物学系和 弗吉尼亚联邦大学弗吉尼亚校区医学院的免疫学 大学自1988年以来一直维护和使用FACScan流式细胞仪，并 已广泛使用该设备(随附文件)。这台仪器是 现在13岁了，需要替补。此外，更替 (FACSCaliber)有一个额外的激光器，可以进行四种颜色的分析。这个 大量使用以及额外容量的综合影响导致 这是购买FACS口径的资金申请。现有FACScan 也将作为后备加溢流仪保存。用法是这样的，它 往往很难在乐器上获得时间。请求的单位 包括4色细胞仪，外加Macintosh G4计算机/显示器PLUS Celiquest软件外加用于存储和打印文件的外围设备。用户 包括培训和两年保修。我们的小组由10个基金组成 研究人员(NIH和一名NSF)积极利用流式细胞术和 有很多出版物都在使用这项技术。此外，该单位是 MCV/NCU的其他用户也可在以下时间按小时收费 适当的培训。目前，我们还有另外七个实验室使用 乐器的使用频率较低。

DESCRIPTION (provided by applicant): The Department of Microbiology and Immunology at the Medical College of Virginia Campus of Virginia Commonwealth University has maintained and operated a FACScan flow cytometer since 1988 and has used this equipment extensively (documentation attached). The instrument is now 13 years old and is in need of replacement. In addition, the replacement (FACScaliber) has an additional laser making possible four color analyses. The combination effect of heavy usage along with additional capacities, leads to this request for funds for purchase of the FACScaliber. The existing FACScan will be also kept as a backup plus overflow instrument. Usage is such that it is frequently difficult to obtain time on the instrument. The requested unit includes the 4 color cytometer, plus a Macintosh G4 computer/monitor plus Celiquest software plus peripherals for storage and print of files. User training and 2 year warranty is included. Our group consists of 10 funded investigators (NIH and one NSF) who are actively utilizing flow cytometry and have a number of publications using this technology. In addition, the unit is also available on a charge per hour basis to other users at MCV/NCU after appropriate training. At present, we have seven other laboratories that use the instrument on a less frequent basis.