Macromolecular Crystallography Data Collection Instrumen

高分子晶体数据采集仪

• 批准号: 6440840
• 负责人: Hwai-Chen Guo
• 金额: $ 46.5万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440840
• 关键词: X ray crystallography; biomedical equipment purchase; structural biology

Funds are requested for a state-of-the-art macromolecular crystallography data collection instrument. We are specifically interested in purchasing an integrated system with a Rigaku RU- H3RHB X-ray generator, a confocal optical system (blue configuration), an inverse phi axis, an MSC R-Axis IV++ area detector, an X-stream cryogenic system, and a control computer with software to drive the instrument and process diffraction data. This new system will directly and immediately impact NIH funded projects among 8 research groups by ensuring adequate X-ray beamtime for crystal screening/characterization and complete data collections. It will complement our 9 year old R-Axis II system during downtime that is increasingly required for maintenance and repairs of this older model, and accommodate our growing need for the instrument. The expanded capabilities in macromolecular crystallographic data collection are vital to pursue the following projects: a) autoproteolysis through N -> O acyl shift, b) N-terminal nucleophile hydrolase, c) MspI as a new structural class of restriction enzymes, d) annexin crystal structures and membrane interactions, e) metal dependent complexes in blood coagulation, f) efflux-mediated resistance to tetracyclines, g) novel antioxidant mechanisms of surfactant proteins, h) structural and mechanistic studies of phosphonatase, i) deciphering the cellular functions of aldolase, j) enzymes in the meta-fission pathway, k) photoprotein aequorin, 1) multiple antibiotic resistance repressor MarR, m) nucleosome assembly chaperones, n) the apoptosome and its role in programmed cell death, o) lipoprotein structure and apoprotein conformation, p) ganglioside GM1-cholera toxin, q) LDL receptor-LDL complex, r) insulin receptor-insulin complex, s) human apolipoprotein C-1. Overall, these projects will apply macromolecular crystallography to enhance our understanding of the function and assembly of biomolecular complexes and cellular machines. These macromolecular assemblies play important roles in enzymology, signalling through membranes, and chromatin assembly. Furthermore, these studies will provide insights into glycoprotein function as well as cellular processes of lipid metabolism mediated by LDL and the formation of both normal and aberrant structures with medical relevance to atherosclerosis and AGU genetic disease.

请求提供资金，用于购置最先进的大分子晶体学数据收集仪器。我们特别有兴趣购买一个集成系统，包括理学RU-H3 RHB X射线发生器，共焦光学系统（蓝色配置），逆phi轴，MSC R-Axis IV++区域探测器，X-stream低温系统，以及带有软件的控制计算机，用于驱动仪器和处理衍射数据。这一新系统将直接和立即影响NIH资助的8个研究小组的项目，确保足够的X射线束时间进行晶体筛选/表征和完整的数据收集。它将在停机期间补充我们使用了9年的R-Axis II系统，这是对这一旧型号的维护和维修越来越多的要求，并满足我们对仪器不断增长的需求。大分子晶体学数据收集能力的扩大对于开展以下项目至关重要：a）通过N -> O酰基转移的自蛋白水解，B）N-末端亲核水解酶，c）作为限制酶的新结构类别的MspI，d）膜联蛋白晶体结构和膜相互作用，e）血液凝固中的金属依赖性复合物，f）流出物介导的对四环素的抗性，g）表面活性剂蛋白的新的抗氧化机制，h）磷酸酶的结构和机理研究，i）解释醛缩酶的细胞功能，j）亚分裂途径中的酶，k）发光蛋白水母发光蛋白，1）多抗生素抗性阻遏物马尔R，m）核小体组装分子伴侣，n）核糖体及其在程序性细胞死亡中的作用，o）脂蛋白结构和载脂蛋白构象，p）神经节苷脂GM 1-霍乱毒素，q）LDL受体-LDL复合物，r）胰岛素受体-胰岛素复合物，s）人载脂蛋白C-1。总的来说，这些项目将应用大分子晶体学来增强我们对生物分子复合物和细胞机器的功能和组装的理解。这些大分子组装在酶学、膜信号传导和染色质组装中起着重要作用。此外，这些研究将提供深入了解糖蛋白功能以及LDL介导的脂质代谢的细胞过程，以及与动脉粥样硬化和AGU遗传疾病具有医学相关性的正常和异常结构的形成。