CORE--DNA ANALYSIS

核心——DNA分析

• 批准号: 6469211
• 负责人: H LEE WEITH
• 金额: $ 21.7万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6469211
• 关键词: DNA; biomedical equipment resource; biomedical facility; computer assisted sequence analysis; gel electrophoresis; neoplasm /cancer; nucleic acid sequence; oncology; personal computers; polymerase chain reaction

The objective of the DNA Analysis Shared Resource is to provide Purdue cancer center researchers the following services: I) automated determination of DNA sequences by fluorescence-based dideoxy sequencing methods using Pharmacia ALF Express sequencing equipment and 2) computer analysis of nucleic acid and protein sequences using mainly the Genetic Computer Group (GCG) suite of software operating on a cluster of Digital Equipment Corporation Mfa workstations. Both of these services have proved to be invaluable resources for cancer research and are used extensively by members of the Cell Growth and Differentiation Program Area. Cancer researchers in the Experimental Therapeutics and Diagnostics Program Area and the Structural Biology Program Area also frequently utilize these resources. DNA Sequencing Facility provides state of the art enhanced DNA sequencing capabilities using flourescent labeled primers or internal fluorescent labeling and standard oligonucleotide primers. The DNA templates and unlabeled primers are provided by the individual investigators. The sequencing reactions, gel electrophoretic analysis, and final editing of ambigious sequence calls are performed by DNA Sequencing Lab personnel. The Computational Biochemistry Laboratory provides specialized computing hardware and software for the Purdue cancer research community. The principal activities in the computational facility are analysis of nucleic acid and protein sequences by data base searching, sequence comparison and alignment, pattern recognition, and secondary structure prediction. The shared resource provides enhanced access to all major sequence databases and the Brookhaven crystal structure database via network connections. In addition to providing network access to powerful computer workstations and software, the facility provides training and advice on most aspects of molecular sequence analysis and also provides customized programming when novel sequence analysis questions are posed by cancer researchers. Access to this expertise is a highly valued feature of the Shared Resource.

DNA分析共享资源的目标是为普渡癌症中心的研究人员提供以下服务：1）使用Pharmacia ALF Express测序设备，通过基于荧光的双脱氧测序方法自动测定DNA序列; 2）主要使用在Digital Equipment Corporation Mfa工作站集群上运行的Genetic Computer Group（GCG）软件套件，对核酸和蛋白质序列进行计算机分析。这两项服务已被证明是癌症研究的宝贵资源，并被细胞生长和分化计划领域的成员广泛使用。实验治疗学和诊断学计划领域和结构生物学计划领域的癌症研究人员也经常利用这些资源。DNA测序设施使用荧光标记引物或内部荧光标记和标准寡核苷酸引物提供最先进的增强型DNA测序能力。DNA模板和未标记引物由研究者个人提供。由DNA测序实验室人员进行测序反应、凝胶电泳分析和最终编辑可疑序列调用。计算生物化学实验室为普渡癌症研究社区提供专业的计算硬件和软件。计算设施的主要活动是通过数据库搜索、序列比较和比对、模式识别和二级结构预测来分析核酸和蛋白质序列。共享资源通过网络连接提供对所有主要序列数据库和Brookhaven晶体结构数据库的增强访问。除了提供强大的计算机工作站和软件的网络访问，该设施提供分子序列分析的大多数方面的培训和建议，并提供定制的编程时，癌症研究人员提出新的序列分析问题。获得这种专业知识是共享资源的一个非常有价值的功能。