Core--Macromolecular crytallography

核心--高分子晶体学

• 批准号: 6469212
• 负责人: JEFFREY T BOLIN
• 金额: $ 21.7万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6469212
• 关键词: X ray crystallography; biomedical equipment resource; biomedical facility; neoplasm /cancer; oncology

The analysis of macromolecular structures by X-ray crystallography provides data critical for an understanding of the beneficial and adverse functions of biological macromolecules and assemblies associated with cancer, which is the focus of research in the Cancer Center's Structural Biology Program. Current studies embrace a wide range of crystallographic research pertaining to basic processes associated with cancer as well as to its prevention and treatment. As a new initiative of the Purdue Cancer Center, the establishment of a Macromolecular Crystallography Shared Resource Core Facility will promote a strong program of cancer-related crystallographic research as well as stimulate new research and additional cross-program collaborations. The essential service of the Resource will be to provide advanced facilities for macromolecular crystallography and experienced technical support to maintain these facilities in superb working condition for direct use by Cancer Center researchers. The proposed shared resource incorporates the extensive existing shared assets of the Structural Biology Program Area and provides access to all Cancer Center members. The essential facilities include laboratories equipped for X-ray diffraction experiments and for the crystallization of macromolecules. Key diffraction instruments include two I2kW rotating anode X-ray generators and three imaging plate diffractometers equipped with focusing mirror optics and cryogenic crystal cooling devices. Equally critical to the success of the shared resource is the involvement of very experienced support personnel. The X-Ray diffraction equipment which will be incorporated into this resource is currently heavily and exclusively used by Cancer Center members.

X射线结晶学对大分子结构的分析为了解与癌症相关的生物大分子和组件的有益和不利功能提供了至关重要的数据，这是癌症中心结构生物学计划的研究重点。目前的研究包括与癌症相关的基本过程以及预防和治疗的广泛的结晶学研究。作为普渡癌症中心的一项新倡议，建立大分子结晶学共享资源核心设施将促进与癌症相关的结晶学研究的强大计划，并刺激新的研究和更多的跨计划合作。该资源的基本服务将是为大分子结晶学提供先进的设施和经验丰富的技术支持，以保持这些设施处于一流的工作状态，供癌症中心的研究人员直接使用。拟议的共享资源结合了结构生物学计划领域广泛的现有共享资产，并提供对所有癌症中心成员的访问。基本设施包括为X射线衍射实验和大分子结晶配备的实验室。主要的衍射仪包括两台I2kW旋转阳极X射线发生器和三台配有聚焦镜光学装置和低温晶体冷却装置的成像板衍射仪。共享资源的成功同样重要的是非常有经验的支助人员的参与。将被纳入这一资源的X射线衍射设备目前主要由癌症中心成员专用。