STRUCT BIOLOGY OF METALLOPROTEINS & BIODEGRADATION OF PCBS & RELATED COMPOUNDS

实验的目的是确定晶体结构 BphD和联苯双加氧酶的初级相确定。 芳烃好氧生物降解的上游途径 化合物通常涉及四个初始步骤：（1）二羟基化 （2）通过双加氧酶使环发生环化反应以形成顺式-二氢二醇产物; 脱氢以形成二羟基芳族产物;（3）环 通过双加氧酶裂解;（4）环分裂产物的水解 丝氨酸水解酶的作用 我们正在研究 催化多氯联苯生物降解步骤的酶 （多氯联苯），目的是了解 生物化学和开发细菌菌株， 它们降解各种PCB的能力。 这门科学是 适用于相对大量的同源途径， 降解另一种异生物质芳香族化合物的健康， 环境或工业意义。 使用BioCARS Station 14-BM-D使用MAD技术。

The aim of the experiment is to determine the crystal structures of BphD and biphenyl dioxygenase with primary phase detennination. The upstream pathway for the aerobic biodegradation of aromatic compounds typically involves four initial steps: (1) dihydroxylation of a ring by a dioxygenase to form a cis-dihydrodiol product; (2) dehydrogenation to form a dihydroxy aromatic product; (3) ring cleavage by a dioxygenase; (4) hydrolysis of the ring-fission product by a serine hydrolase. We are studying the structure and function of the enzymes that catalyze these steps in the biodegradation of PCBs (polychlorinated biphenyls) with the goal of understanding the biochemistry and developing bacterial strains that are enhanced in their ability to degrade the full range of PCBs. This science is applicable to a relatively large number of homologous pathways for the degradation of another xenobiotic aromatic compounds of health, environmental, or industrial significance. Use of BioCARS Station 14-BM-D using MAD technique.