CORE--MOLECULAR CYTOGENETICS FACILITY

描述(申请人提供)：分子细胞遗传学核心设施 为癌症中心研究人员提供细胞遗传学和分子DNA分析 新鲜细胞和组织，已建立的和体细胞系，人类肿瘤和 小鼠肿瘤，特别是来自转基因小鼠的肿瘤。此核心是新的，因为 上一次提交的这项癌症中心拨款是在 方案1、3、5和6成员的请求。细胞遗传学服务包括 成纤维细胞、淋巴母细胞或体细胞的常规Giemsa(G)显带和C显带 用于确定核型的细胞杂交系。这项服务还包括 包括利用荧光原位杂交(FISH)来更好地 澄清这些核型，包括m鱼(多色鱼)和 SKY(光谱核型分析)，使用24种不同的颜色进行分析 染色体的变化。FISH也被用来研究人类染色体的变化 肿瘤(尤其是石蜡切片)、骨髓中的间期细胞 移植，以及基因的染色体定位。分子服务 这一核心包括对肿瘤的微卫星分析 肿瘤的杂合性、突变分析和骨的嵌合体研究 骨髓移植。在某些情况下，为了执行服务，组织培养 需要的服务包括：总体增长、维护和冻结 病人？细胞系；淋巴细胞的转化以产生永久性的 淋巴母细胞系；从细胞系和外周血中制备DNA； 并根据需要产生体细胞系。在其他情况下， 提供包括比较基因组学在内的新的、专业化的服务 杂交(CGH)和嵌合体分析已经发展起来。这些 由于增加了两台计算机辅助的计算机，分析工作得到了便利 显微镜装置和DNA测序仪。为响应用户请求， FILITY积极开发分子细胞遗传学新技术。

DESCRIPTION (provided by applicant): The Molecular Cytogenetics Core Facility provides Cancer Center investigators cytogenetic and molecular DNA analysis of fresh cells and tissue, established and somatic cell lines, human tumors and mouse tumors, particularly those from transgenic mice. This Core is new since the last submission of this Cancer Center Grant and was developed at the request of members of Programs 1, 3, 5 and 6. Cytogenetic services consist of routine Giemsa (G)-banding and C-banding on fibroblast, lymphoblast or somatic cell hybrid lines for the purpose of determining karyotypes. The service also includes utilization of fluorescence in situ hybridization (FISH) for better clarification of these karyotypes, including both m-FISH (multicolor FISH) and SKY (spectral karyotyping), that uses 24 different colors to analyze chromosomal changes. FISH is also utilized to study chromosomal changes in interphase cells in tumors (especially in paraffin sections), in bone marrow transplants, and for chromosomal localization of genes. The molecular service of this Core consists of microsatellite analysis of tumors for loss of heterozygosity, mutation analysis of tumors, and chimerism studies of bone marrow transplants. In some instances to perform the services, tissue culture services are needed and include: general growth, maintenance, and freezing of patients? cell lines; transformation of lymphocytes to generate permanent lymphoblast lines; preparation of DNA from cell lines and peripheral blood; and generation of somatic cell lines as needed. In other instances, to perform the services, new, specialized services including comparative genomic hybridization (CGH) and chimerism analysis have been developed. These analyses have been facilitated by the addition of two computer-assisted microscopy units and a DNA sequencer. In response to user requests, the facility actively develops new technology in molecular cytogenetics.