Helicobacter pylori - cellular immunity and protection against multiple sclerosis

幽门螺杆菌 - 细胞免疫和预防多发性硬化症

• 批准号: 1833939
• 金额: --
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1833939
• 关键词: Helicobacter; pylori; cellular; immunity; protection

Multiple sclerosis (MS) is a demyelinating autoimmune disorder affecting the central nervous system (CNS), where inflammatory T-helper 1 (Th1) and Th17 cell responses damage the myelin sheath of neural axons and cause neurodegeneration. The balance between these cells and anti-inflammatory regulatory T-cell (Treg) responses is important in MS development and progression.H. pylori is a bacterial pathogen that colonises the human stomach from childhood. It is the major cause of peptic ulceration and gastric cancer, however it also induces a potent Treg response and beneficial roles have been described. We were the first to demonstrate protective effects of H. pylori against MS in a mouse model, and reported a lower prevalence of H. pylori amongst MS patients.This project will evaluate the influence of H. pylori on MS, and investigate the immunological mechanisms. H. pylori status of MS patients will be ascertained by serology, and epidemiological analyses will compare the course of MS in infected and uninfected patients (including disability scores, relapse rates, and responsiveness to therapies). Using flow cytometry and RT-qPCR, we will quantify peripheral blood Treg, Th1 and Th17 cells and investigate differences in their homing receptor expression for migration to the gastric mucosa rather than the CNS.

多发性硬化症（MS）是一种影响中枢神经系统（CNS）的脱髓鞘自身免疫性疾病，其中炎性辅助性T细胞1（Th 1）和Th 17细胞反应损害神经轴突的髓鞘并引起神经变性。这些细胞和抗炎调节性T细胞（Treg）反应之间的平衡在MS发展和进展中是重要的。幽门螺杆菌是一种细菌病原体，从儿童时期就在人类胃中定植。它是消化性溃疡和胃癌的主要原因，但它也诱导了有效的Treg反应，并且已经描述了有益的作用。我们是第一个证明H. pylori在小鼠模型中对MS的作用，并报告了较低的H. pylori感染对MS患者的影响。pylori对MS的影响，并探讨其免疫学机制。H.将通过血清学确定MS患者的幽门螺杆菌状态，流行病学分析将比较感染和未感染患者的MS病程（包括残疾评分、复发率和对治疗的反应性）。使用流式细胞术和RT-qPCR，我们将定量外周血Treg，Th 1和Th 17细胞，并研究其归巢受体表达迁移到胃粘膜而不是CNS的差异。