Regulatory circuitry controlling the cell cycle of Helicobacter pylori
控制幽门螺杆菌细胞周期的调节电路
基本信息
- 批准号:9207987
- 负责人:
- 金额:$ 14.8万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2017
- 资助国家:美国
- 起止时间:2017-09-01 至 2020-08-31
- 项目状态:已结题
- 来源:
- 关键词:ATP phosphohydrolaseAntibioticsBacteriaBindingBinding ProteinsBiological AssayBiological ModelsCancer EtiologyCaulobacter crescentusCell CycleCell Cycle ProgressionCell ProliferationCell SeparationCellsCessation of lifeChIP-seqChromosome SegregationChromosomesCytokinesisDNADNA biosynthesisDataDevelopmentDrug TargetingFDA approvedFoundationsFrequenciesGenesGenetic TranscriptionGoalsGrowthGrowth and Development functionHelicobacter pyloriHumanImmunoprecipitationKnowledgeLife Cycle StagesMass Spectrum AnalysisMissionMolecularMolecular MachinesNucleotidesOutcomePatientsPeptic UlcerPharmaceutical PreparationsPreventiveProteinsProteolysisPublic HealthRegulonReplication InitiationReplication OriginResearchRisk FactorsSamplingSiteTechniquesTestingTherapeuticTranslationsUnited States National Institutes of HealthWorkantimicrobial drugcdc Genescell growthcellular developmentchromatin immunoprecipitationchromosome replicationcrosslinkdeep sequencingevent cyclein vivoinnovationmalignant stomach neoplasmnew therapeutic targetnovelpathogenpromoterprotein complextooltranscription factor
项目摘要
Project Summary/Abstract
There is a fundamental gap in our understanding of how helicobacter pylori (Hp), a bacterium that is highly
relevant to public health, regulates its life cycle. Although mechanistic details of how Hp induces damage to
host cells have been revealed, our understanding for how this pathogen regulates its own proliferation remains
unclear. The existence of this knowledge gap represents a burden in the need to develop novel specie-specific
drugs that can control the proliferation of Hp in human patients. Our long-term goal is to identify drug targets
that are essential in regulating Hp cell cycle and proliferation. The overall objective of the proposed research is
to define the regulatory circuitry that controls the forward progression of Hp cell cycle. Our central hypothesis is
that the chromosome replication initiator DnaA in Hp is a central node connecting multiple cell cycle regulating
factors. DnaA is a multifunctional protein that aside from opening the chromosomal region known as the origin
of replication (ori), it also acts as a highly regulated transcription factor. The rationale for the proposed
research is that DNA replication initiators have been shown to influence the activity of other cellular machines
involved in the cell cycle, such as cytokinesis, cell growth, and cellular development. Our plan is to test our
central hypothesis, and thus accomplish our overall objective for this project, by pursuing the following three
specific aims: 1) Define the DnaA transcriptional regulon by analyzing the global chromosome profile of
promoters bound by DnaA using chromatin immunoprecipitation assays followed by deep sequencing; 2)
Identify regulators that modulate DnaA's activity at the origin of replication by cross-linking and isolating the
chromosomal ori-proteins complex using a DNA-sampling technique; 3) Identify factors that directly interact
with DnaA by isolating DnaA-containing protein complexes within the cell. Successful completion of the
proposed research is expected to vertically advance and expand our knowledge of and ability to assemble the
regulatory circuitry that controls Hp cell cycle. Furthermore, findings from our work will broaden our
understanding of how the highly conserved replication initiators coordinate DNA synthesis with the progression
of the cell cycle. Our proposed research is innovative, in our opinion, because it represents a substantive
departure from the status quo by targeting Hp-specific cell cycle regulators as targets for new antibiotics.
项目总结/文摘
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Paola E Mera其他文献
Chromosome dynamics in bacteria: triggering replication at opposite location and segregation in opposite direction
细菌中的染色体动力学:触发相反位置的复制和相反方向的分离
- DOI:
- 发表时间:
2019 - 期刊:
- 影响因子:0
- 作者:
Ady B. Meléndez;Inoka P. Menikpurage;Paola E Mera - 通讯作者:
Paola E Mera
The B12 receptor BtuB alters the membrane integrity of Caulobacter crescentus.
B12 受体 BtuB 改变新月柄杆菌的膜完整性。
- DOI:
10.1099/mic.0.000753 - 发表时间:
2019 - 期刊:
- 影响因子:1.5
- 作者:
Inoka P. Menikpurage;D. Barraza;Ady B. Meléndez;Sierra Strebe;Paola E Mera - 通讯作者:
Paola E Mera
Discovering and Applying the Urban Rules of Life to Design Sustainable and Healthy Cities.
发现并应用城市生活规则来设计可持续和健康的城市。
- DOI:
- 发表时间:
2021 - 期刊:
- 影响因子:2.6
- 作者:
T. A. Langen;C. Cannon;D. Blackburn;E. Morgan;Paola E Mera - 通讯作者:
Paola E Mera
Chromosome Dynamics in Bacteria: Triggering Replication at the Opposite Location and Segregation in the Opposite Direction
细菌中的染色体动力学:触发相反位置的复制和相反方向的分离
- DOI:
10.1128/mbio.01002-19 - 发表时间:
2019 - 期刊:
- 影响因子:6.4
- 作者:
Ady B. Meléndez;Inoka P. Menikpurage;Paola E Mera - 通讯作者:
Paola E Mera
Paola E Mera的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Paola E Mera', 18)}}的其他基金
Deciphering the molecular circuitry that controls cell cycle progression in bacteria
破译控制细菌细胞周期进程的分子电路
- 批准号:
10478051 - 财政年份:2019
- 资助金额:
$ 14.8万 - 项目类别:
Deciphering the molecular circuitry that controls cell cycle progression in bacteria
破译控制细菌细胞周期进程的分子电路
- 批准号:
10227369 - 财政年份:2019
- 资助金额:
$ 14.8万 - 项目类别:
Deciphering the molecular circuitry that controls cell cycle progression in bacteria
破译控制细菌细胞周期进程的分子电路
- 批准号:
10274049 - 财政年份:2019
- 资助金额:
$ 14.8万 - 项目类别:
Deciphering the molecular circuitry that controls cell cycle progression in bacteria
破译控制细菌细胞周期进程的分子电路
- 批准号:
10678741 - 财政年份:2019
- 资助金额:
$ 14.8万 - 项目类别:
Deciphering the molecular circuitry that controls cell cycle progression in bacteria
破译控制细菌细胞周期进程的分子电路
- 批准号:
10249364 - 财政年份:2019
- 资助金额:
$ 14.8万 - 项目类别:
Deciphering the molecular circuitry that controls cell cycle progression in bacteria
破译控制细菌细胞周期进程的分子电路
- 批准号:
10468560 - 财政年份:2019
- 资助金额:
$ 14.8万 - 项目类别:
Deciphering the molecular circuitry that controls cell cycle progression in bacteria
破译控制细菌细胞周期进程的分子电路
- 批准号:
10700095 - 财政年份:2019
- 资助金额:
$ 14.8万 - 项目类别:
Deciphering the molecular circuitry that controls cell cycle progression in bacteria
破译控制细菌细胞周期进程的分子电路
- 批准号:
9797583 - 财政年份:2019
- 资助金额:
$ 14.8万 - 项目类别:
The Spatial and Temporal Integration of Chromosome Segregation and Cytokinesis
染色体分离和细胞分裂的时空整合
- 批准号:
8125755 - 财政年份:2011
- 资助金额:
$ 14.8万 - 项目类别:
The Spatial and Temporal Integration of Chromosome Segregation and Cytokinesis
染色体分离和细胞分裂的时空整合
- 批准号:
8307562 - 财政年份:2011
- 资助金额:
$ 14.8万 - 项目类别:
相似海外基金
DYNBIOTICS - Understanding the dynamics of antibiotics transport in individual bacteria
DYNBIOTICS - 了解抗生素在单个细菌中转运的动态
- 批准号:
EP/Y023528/1 - 财政年份:2024
- 资助金额:
$ 14.8万 - 项目类别:
Research Grant
Engineering Streptomyces bacteria for the sustainable manufacture of antibiotics
工程化链霉菌用于抗生素的可持续生产
- 批准号:
BB/Y007611/1 - 财政年份:2024
- 资助金额:
$ 14.8万 - 项目类别:
Research Grant
Hitting bacteria with a Bam: Lectin-Like Antimicrobials as New Antibiotics
用 Bam 击中细菌:凝集素类抗菌剂作为新型抗生素
- 批准号:
DP230102150 - 财政年份:2023
- 资助金额:
$ 14.8万 - 项目类别:
Discovery Projects
Systematic identification of synthetic interactions in bacteria towards the next-generation of antibiotics
系统鉴定细菌与下一代抗生素的合成相互作用
- 批准号:
468567 - 财政年份:2022
- 资助金额:
$ 14.8万 - 项目类别:
Operating Grants
“L-form” bacteria: basic science, antibiotics, evolution and biotechnology
L 型细菌:基础科学、抗生素、进化和生物技术
- 批准号:
FL210100071 - 财政年份:2022
- 资助金额:
$ 14.8万 - 项目类别:
Australian Laureate Fellowships
Repurposing Gram-positive Antibiotics for Gram-Negative Bacteria using Antibiotic Adjuvants
使用抗生素佐剂重新利用革兰氏阳性抗生素治疗革兰氏阴性菌
- 批准号:
10708102 - 财政年份:2022
- 资助金额:
$ 14.8万 - 项目类别:
Repurposing Gram-positive Antibiotics for Gram-Negative Bacteria using Antibiotic Adjuvants
使用抗生素佐剂重新利用革兰氏阳性抗生素治疗革兰氏阴性菌
- 批准号:
10587015 - 财政年份:2022
- 资助金额:
$ 14.8万 - 项目类别:
Isolation, identification and characterization of potentially novel antibiotics from rhizospheric bacteria without detectable in vitro resistance
从根际细菌中分离、鉴定和表征潜在的新型抗生素,且体外未检测到耐药性
- 批准号:
10581945 - 财政年份:2021
- 资助金额:
$ 14.8万 - 项目类别:
Isolation, identification and characterization of potentially novel antibiotics from rhizospheric bacteria without detectable in vitro resistance
从根际细菌中分离、鉴定和表征潜在的新型抗生素,且体外未检测到耐药性
- 批准号:
10358855 - 财政年份:2021
- 资助金额:
$ 14.8万 - 项目类别:
Developing novel antibiotics from natural products against resistant bacteria
从天然产物中开发针对耐药细菌的新型抗生素
- 批准号:
2599490 - 财政年份:2021
- 资助金额:
$ 14.8万 - 项目类别:
Studentship














{{item.name}}会员




