Developmental Differences in Brain Androgen Synthesis

大脑雄激素合成的发育差异

• 批准号: 6446584
• 负责人: SARAH E LONDON
• 金额: $ 2.31万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6446584
• 关键词: RNase protection assay; androgens; aromatase; biological polymorphism; complementary DNA; developmental genetics; developmental neurobiology; estrogens; gender difference; gene expression; genetic library; genetic polymorphism; genetic promoter element; genetic regulation; genetic screening; genome; hydrolase; in situ hybridization; messenger RNA; neuroanatomy; neurogenesis; neurohormones; songbirds; steroid hormone biosynthesis; telencephalon; transcription factor

DESCRIPTION (provided by applicant): The zebra finch song system consists of sexually dimorphic nuclei. Early estrogen treatment masculinizes telencephalic song nuclei in genetic females, but blocking estrogen does not demasculinize these nuclei in genetic males. Studies have suggested that the existence of a genetic factor intrinsic to the brain underlies the masculine pattern of song system organization. Recently, a sex difference has been seen in transcriptional levels of 17 alpha hydroxylase (CYP 17), the enzyme that makes androgen, in the telencephalon of developing zebra finches. It is hypothesized that GYP 17 expression extends through the steroid sensitive period of song system development, and is distributed near the song nuclei. This will be investigated using in situ hybridization. It is hypothesized that the different levels of CYP17 mRNA reflect sex differences in protein-mediated transcriptional regulation. DNA sequence from the promoter region of the CYP 17 gene will be determined by screening a cDNA library, performing 5? RACE, and screening a zebra finch genomic library. This represents a first step in identifying the factors that differentially regulate CYP 17 transcription in the telencephalon of developing male and female zebra finch. They will also indicate potential regulatory mechanisms for androgen synthesis in other species, including humans.

描述（由申请人提供）：斑胸草雀的鸣叫系统包括