Custom integration tools for functional genomics

描述（由申请人提供）：该提案开发了强大的新 在预定的温度下获得有效的位点特异性整合的方法 基因组序列这部作品详细阐述了我的一系列成功的研究成果。 实验室，涉及在它们的位置由噬菌体整合酶介导的重组 紧凑的（30 - 40-bp）attB和attP识别位点。我们已经证明 该反应代表了最有效的位点特异性整合系统 为更高的细胞而开发。在拟议的战略中， 位于引入的DNA载体上存在的attB位点和 位于小鼠基因组中的天然存在的attP样序列。结果 是将进入的DNA共价整合到预先指定的位置， 老鼠的基因组噬菌体整合酶的定向进化被用于产生一种 具有前所未有的效率和专用性的大型集成工具库。 有了这个系统，可以为任何基因组设计定制的整合工具， 序列，并用于在高水平下整合大或小尺寸的DNA插入物。 效率要插入的DNA可以根据需要定制设计。因为 该方法是特定于站点的，插入的位置自动 知道的我们开发合适的DNA改组和遗传筛选方法 这里用于生成定制集成工具库。该战略是 可扩展到小鼠基因组中的基本上所有基因。

DESCRIPTION (provided by applicant): This proposal develops powerful new methods for obtaining efficient site-specific integration at pre-determined genomic sequences. The work elaborates on a successful line of research in my laboratory involving recombination mediated by phage integrases at their compact (30 - 40-bp) attB and attP recognition sites. We have demonstrated that this reaction represents the most efficient site-specific integration system developed to date for higher cells. In the proposed strategy, integration takes place between an attB site present on an incoming DNA vector and a naturally-occurring attP-like sequence located in the mouse genome. The result is covalent integration of the incoming DNA into a pre-specified position in the mouse genome. Directed evolution of phage integrases is used to produce a large library of integration tools of unprecedented efficiency and specificity. With this system, a custom integration tool can be designed for any genomic sequence and used to integrate a DNA insert of large or small size at high efficiency. The DNA to be inserted can be custom designed as desired. Because the method is site-specific, the location of the insertion is automatically known. We develop the appropriate DNA shuffling and genetic screening methods here for generation of a library of custom integration tools. The strategy is scalable to reach essentially all genes in the mouse genome.