GENETIC DISSECTION OF MURINE PERIODONTAL WOUND HEALING

牙周组织，覆盖和支撑牙齿的组织， 包括牙槽骨、牙根牙骨质、牙周韧带和牙龈。是 一般认为牙周病的病因是传染性的， 然而，宿主的变异允许一系列疾病的表达。 抑制疾病过程导致不可逆的组织损失， 后期的畸形和语音障碍。方法来 牙周病的治疗包括消除感染。 然而，这可能无法恢复必要的形式和功能。这些牙周 表现出牙槽骨、牙周韧带和 牙龈应考虑重建治疗或涉及的牙齿 被截肢并被假肢取代。然而，由于最近的进展， 在基因工程中，还有另一种可能性。如果你知道这些因素 参与组织再生，这些因子可以被输送并再生 允许的.动物模型提供了组织学观察的机会， 可能有助于增强对以下愈合生物学的理解 重建程序。在提案中，调查人员描述了一种独特的 具有确定遗传基础的组织再生小鼠模型。发表 模型中的观察结果包括软骨和皮肤的正常替换。 他们将在这只老鼠身上使用一种已建立的实验性牙周缺损 1）确定再生是否实际发生，如果是，则推进 了解导致森林再生的时间和空间事件 牙周组织，2）确定愈合部位的基因表达，并分析 潜在的分子，以促进愈合，和3）确定是否同源小鼠， 已经选择了参与耳组织再生的基因 也显示牙周愈合。

The periodontium, the tissues investing and supporting teeth, includes aveolar bone, root cementum, periodontal ligament, and gingiva. It is generally accepted that the etiology of periodontal disease(s) is infectious in origin, however, variations in the host allow a range of disease expression. Invariably the disease process results in irreversible tissue loss resulting in disfiguration and phonetic imparement in its advanced stages. Approaches to therapy of periodontal disease(s) include elimination of the infection. However, this may not restore necessary form and function. These periodontal defects exhibiting crippling loss of alveolar bone, periodontal ligament, and gingiva should be considered for reconstructive therapy or the involved teeth be amputated and replaced by a prosthesis. However, due to the recent advances in genetic engineering, there is another possibility. If one knows the factors involved in regrowth of tissue, those factors could be delivered and regrowth allowed. Animal models offer the opportunity for histologic observations, which may contribute, to enhanced understanding of the biology of healing following reconstructive procedures. In the proposal, the investigators describe a unique mouse model of tissue regeneration with a defined genetic basis. Published observations in the model include the normal replacement of cartilage and skin. They will use an established experimental periodontal defect in this mouse model to 1) determine if regeneration actually occurs and if so, advance the understanding of temporal and spatial events leading to regeneration of the periodontium, 2) determine gene expression at the site of healing and analyze potential molecules to enhance healing, and 3) determine if congenic mice which have already been selected for the genes involved in regeneration of ear tissue also display periodontal healing.