Microscopy Facility

复杂细胞结构的成像是现代癌症生物学的核心，也是最先进的 显微镜设备对于癌症中心的使命来说是必不可少的。因此，维斯塔尔研究所做出了一项 大力获取完成其使命所需的工具，并由 非常称职的生物影像专家詹姆斯·海登先生。 显微镜设备为维斯塔尔癌症中心的成员发挥了重要作用 1973年。在过去的五年里，该设施得到了巨大的发展，随着科学重点的变化 以及新技术和仪器的加入，已经演变成研究所的一项主要资产 和癌症中心。有了新的领导，重新配置了空间以提高利用率，新的技术 在各级的应用程序、新的支助服务和培训方面，该机制经历了大量的 癌症中心会员使用量增加。 自2003年以来，所有三个癌症中心研究项目的32个实验室中有31个使用了 由显微镜设施提供的服务。重大科学成就发表在《高影响》杂志上 使用新的设施设备，包括Xenogen MS成像系统，已经实现了期刊 双光子显微系统对肿瘤转移的活体生物发光研究(Huang，Kissil和Pure) 成像免疫细胞在体内的运动及其与肿瘤细胞的相互作用(Ertl和Weinger 实验室)和活细胞显微镜系统，以成像体外细胞-细胞和细胞-基质相互作用(M。 Herlyn、Heber-Katz和Pure实验室)。标准广域和共聚焦显微镜(利伯曼、毛尔 和Rauscher实验室)也为分子相互作用的研究提供了帮助 亚细胞水平。自上次续签以来，训研所已承付约514 000美元用于 新的仪器设备和升级。

The imaging of complex cellular structures is central to modern cancer biology, and a state-of-the-art Microscopy Facility is essential to the mission of a Cancer Center. As such, the Wistar Institute has made a strong effort to acquire the necessary instrumentation to accomplish its mission and to have it managed by a highly competent biological imaging specialist, Mr. James Hayden. The Microscopy Facility has served an important role for the members of the Wistar Cancer Center since 1973. Over the past five years, the Facility has grown tremendously and, with changes in scientific focus and the addition of new technologies and instrumentation, has evolved into a premier asset of the Institute and Cancer Center. With new leadership, a reconfiguration of space that improved utilization, new technical applications, new support services, and training at all levels, the Facility has experienced a substantial increase in Cancer Center member usage. Since 2003, thirty-one out of 32 laboratories from all three Cancer Center research programs have used services provided by the Microscopy Facility. Significant scientific accomplishments published in high impact journals have been achieved using new facility equipment, including the Xenogen MS imaging system for in vivo bioluminescent studies of tumor metastases (Huang, Kissil and Pure), the 2 Photon microscopy system to image in vivo movement of immune cells and their interaction with tumor cells (Ertl and Weninger laboratories), and the Live-Cell microscopy system to image in-vitro cell-cell and cell-matrix interactions (M. Herlyn, Heber-Katz, and Pure laboratories). Standard wide-field and confocal microscopy (Lieberman, Maul and Rauscher laboratories) available through the Facility has also aided studies in molecular interactions at the subcellular level. Since the previous renewal, approximately $514,000 was committed by the Institute for new instrumentation and upgrades.