LYSERGIC ACID DIETHYLAMIDE INDUCED GENE EXPRESSION

麦角酸二乙酰胺诱导的基因表达

• 批准号: 6362806
• 负责人: CHARLES D NICHOLS
• 金额: $ 4.28万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6362806
• 关键词: RNase protection assay; antibody formation; gene expression; hallucinogens; in situ hybridization; inhibitor /antagonist; laboratory rabbit; laboratory rat; lysergic acid diethylamide; neuropharmacology; nucleic acid sequence; receptor expression

The specific aims of the research in this proposal are to identify and characterize genes whose expression patterns are influenced by acute LSD administration in the brains of rats. Candidate genes will be identified from a pool of clones isolated in previous Differential Display PCR experiments. Differential expression within the brain for candidates will be determined and characterized by RNase protection and tissue in situ experiments. Antibodies will be generated to analyze candidate protein expression. Differential expression will be tested after administration of different hallucinogens. The receptor mediated pathways in which the differentially expressed genes act will be determined in experiments employing a variety of receptor antagonists. Ultimately these genes, their products and the pathways that are involved may be investigated for their potential role in animal behavior and in human mental disorders such as schizophrenia. This training and research could provide the basis for future work in establishing an independent research program in an academic environment.

本提案研究的具体目的是鉴定和表征其表达模式受大鼠大脑中急性 LSD 给药影响的基因。候选基因将从先前差异显示 PCR 实验中分离的克隆池中鉴定出来。候选者大脑内的差异表达将通过 RNase 保护和组织原位实验来确定和表征。将生成抗体来分析候选蛋白的表达。施用不同致幻剂后将测试差异表达。差异表达基因发挥作用的受体介导途径将在使用多种受体拮抗剂的实验中确定。最终，这些基因、它们的产物和所涉及的途径可能会被研究，以了解它们在动物行为和人类精神障碍（如精神分裂症）中的潜在作用。这种培训和研究可以为未来在学术环境中建立独立研究项目的工作奠定基础。