STRUCTURE OF ENZYME SUBSTRATE COMPLEX FOR GENERATION OF UDP N ACETYLMURAMIC ACID

产生 UDP N 乙酰胞壁酸的酶底物复合物的结构

• 批准号: 6437582
• 负责人: JAMES M HOGLE
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6437582
• 关键词: bacteria; bioimaging /biomedical imaging; biomaterials; biomedical resource; infection; radiography; structural biology

UDP-N-acetylenolpyruvylglucosamine reductase (MurB) is an enzyme in the peptidoglycan biosynthetic pathway of bacteria. It is responsible for the second committed step, namely the reduction of enolpyruvyl-UDP-N-acetylglucosamine (EP-UDPGlcNAc) to UDP-N-acetylmurammic acid (UDPMurNAc) by NADPH via a tightly bound flavin adenine dinucleotide cofactor. This resulting sugar is then the foundation for the addition of the five amino acids comprising the peptide chain and subsequently will be linked with N-acetylglucosamine to form the repeating disaccharide-pentapeptide subunit. Because of the essential nature of this enzyme in the synthesis of peptidoglycan to maintain osmotic integrity, this enzyme is a novel drug target for design of new antibacterial agents.

UDP-N-乙炔基葡萄糖胺还原酶（MurB）是一种酶， 在细菌的肽聚糖生物合成途径中。 是 负责第二个承诺的步骤，即减少 烯醇化酰基-UDP-N-乙酰葡糖胺（EP-UDPGlcNAc）， NADPH通过紧密结合的UDP-N-乙酰胞壁酸（UDPMurNAc） 黄素腺嘌呤二核苷酸辅因子。 这种糖是 添加五种氨基酸的基础， 肽链，随后将与N-乙酰葡糖胺连接 以形成重复的二硫键肽-五肽亚基。 因为 这种酶在肽聚糖合成中的基本性质 为了保持渗透完整性，这种酶是一种新型药物靶点， 设计新的抗菌剂。