CORE--IMMUNOHISTOCHEMISTRY

核心--免疫组织化学

• 批准号: 6479990
• 负责人: Brian Johnstone
• 金额: $ 3.83万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6479990
• 关键词: biomedical facility; bone; cartilage; histochemistry /cytochemistry; histology; immunocytochemistry; tissue /cell preparation

Histology, histochemistry and immunohistochemistry are extremely valuable tools in the experiment armamentarium of researchers involved in studies of connective tissues and their pathologies. Although these methods are routine for soft tissue samples, examination of tissues such as bone and cartilage is made difficult by their material properties. For example, special techniques are required to process non-decalcified bone for immunohistochemistry such as the use of water soluble, cold polymerizing plastics or the use of special adhesives, supports, and slides after thin sectioning of frozen material on a motorized cryostat. With these techniques one can identify antigens present on the surface of cells, whether in inflammatory infiltrates, in cartilage, bone, synovium, or any other tissue; and non-cellular antigens present within the matrix. To accomplish such analyses requires expertise in technique that individual laboratory personnel usually do not possess. The objective of the Histology/Immunohistochemistry Core of the NEOMAMDC is to continue to function as a shared resource and centralized facility for the processing embedding, cutting, and histological, histochemical and immunohistochemical staining of bone, cartilage and joint-related tissue samples generated as part of the research activities of center personnel. As a core facility, the laboratory provides economy of supplies, equipment and effort, as well as making new technology available to investigators not currently using these techniques. The laboratory will continue to promote interactions between the various investigators in the NEOMAMDC.

组织学、组织化学和免疫组织化学是极其有价值的 参与研究的研究人员的实验工具 结缔组织和它们的病理学。虽然这些方法 软组织样本的常规检查，组织如骨和 软骨由于其材料特性而变得困难。比如说， 需要特殊的技术来处理非脱钙骨， 免疫组化等采用水溶、冷聚合 塑料或使用特殊的粘合剂，支持，和幻灯片后，薄 在电动低温恒温器上对冷冻材料进行切片。与这些 技术可以鉴定存在于细胞表面的抗原， 无论是在炎性浸润中，在软骨、骨、滑膜或任何 其它组织;和存在于基质内的非细胞抗原。到 完成这样的分析需要技术方面的专门知识， 实验室人员通常不具备。的目的 组织学/免疫组织化学NEOMAMDC的核心是继续 作为一种共享资源和集中处理设施， 包埋、切割、组织学、组织化学和 骨、软骨和关节相关组织的免疫组织化学染色 作为中心人员研究活动的一部分而产生的样本。 作为一个核心设施，实验室提供经济的用品，设备 以及为调查人员提供新技术 目前没有使用这些技术。实验室将继续 促进NEOMAMDC各研究者之间的互动。