Tissue Engineered Meniscus Repair

组织工程半月板修复

• 批准号: 7074210
• 负责人: Brian Johnstone
• 金额: $ 20.92万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-15 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7074210
• 关键词: articular cartilage; autologous transplantation; autoradiography; biomaterial development /preparation; biomaterial evaluation; biotechnology; cartilage development; goats; immunocytochemistry; joint disorder; knee; longitudinal animal study; medical implant science; mesenchyme; musculoskeletal injury; musculoskeletal regeneration; radiotracer; stem cells; tissue engineering; tissue support frame; wound healing

DESCRIPTION (provided by applicant): Loss of the meniscus, in part or in total, greatly alters knee function and predisposes the joint to degenerative changes. The goal of this project is the repair or regeneration of critical-sized defects in the medial meniscus to restore meniscal function and prevent joint damage. Mesenchymal progenitor cells are potential repair cells for meniscal defects. In this study, autologous bone marrow-derived mesenchymal progenitor cells will used to create a fibrocartilaginous tissue in vitro that is then implanted for meniscal repair in vivo. The hypothesis to be tested is that tissue-engineered cartilage will provide a superior implant for meniscal repair. This will be tested in two different meniscal repair models: Specific Aim 1: To repair a surgically created mid-substance meniscal tear. The aim is to use a tissue-engineered cartilage to repair a surgically created tear in the medial meniscus. The cartilage will be created in vitro with autologous marrow-derived mesenchymal progenitor cells seeded into a biodegradable scaffold and cultured in a chondrogenic medium. The implant will be sutured into a full-thickness longitudinal tear. The repair potential of this tissue-engineered construct will be compared with those of empty scaffolds and no-implant sutured tears. Specific Aim 2: To regenerate meniscal tissue in a surgically created partial meniscectomy. A large tissue-engineered cartilaginous implant will be used to regenerate a partial meniscectomy of the goat medial meniscus. As for aim 1, the tissue-engineered implant will be composed of the biodegradable scaffold containing autologous mesenchynial progenitor cells, cultured in chondrogenic medium prior to implantation. For both aims, the meniscal implant will be analyzed at 12 weeks and 12 months after implantation. The operated knee joints will be subjected to biomechanical, morphological, histological and immunohistochemical analyses to provide a comprehensive assessment of the success of the tissue-engineering implants in the two repair models.

描述（由申请人提供）：半月板部分或全部丧失，极大地改变了膝关节功能，使关节易发生退行性变化。该项目的目的是修复或再生内侧半月板的临界大小的缺陷，以恢复半月板功能和防止关节损伤。间充质祖细胞是半月板缺损的潜在修复细胞。在这项研究中，自体骨髓来源的间充质祖细胞将在体外产生纤维软骨组织，然后在体内植入半月板修复。需要验证的假设是，组织工程软骨将为半月板修复提供更好的植入物。这将在两种不同的半月板修复模型中进行测试：