CORE--TRANSGENIC ANIMAL

密歇根大学多功能关节炎中心转基因 Animal Model Core为UM-UM-MAC提供基因工程小鼠 investigators.传统的转基因小鼠和突变小鼠 在胚胎干细胞（ES细胞）中通过基因靶向诱导产生用于 investigators.核心组织保证至少有三只转基因小鼠 将为提交的每个转基因构建体产生。核心人员 提供经过质量检测的试剂，并与研究人员广泛合作， 确保基因打靶实验的成功。芯部具有 出色的业绩记录。在前一笔赠款的前四年 期间，从72个DNA构建体中产生了687只转基因小鼠， 10名UM-MAC研究人员的DNA构建体。小鼠诱导突变， 为6名研究者制备了12个基因。值得注意的是， 密歇根大学产生的13只基因靶向小鼠中有12只 已经在UM-MAC研究人员的实验室中产生。研究 UM-MAC调查人员对这些动物的研究导致了许多 关节炎，肌肉骨骼疾病， 炎症和基因调控。我们预计在未来的四年里 UM-MAC调查人员将在 至少有80项转基因小鼠申请和48项生产 生殖系ES细胞-小鼠嵌合体，用于产生具有 新的突变 萨莉·坎珀博士从1986年开始就在她的研究中使用转基因小鼠 并且在该领域中被广泛发表。琳达萨缪尔森博士建立了 年，密歇根大学的胚胎干细胞基因打靶技术 1990. 坎伯博士和萨缪尔森博士将与转基因核心 指导委员会（由经验丰富的用户和UM的一名成员组成） MAC执行委员会）监测转基因核心。资金将 用于确保有高技能的人员来满足 UM-MAC调查员的需求。调查人员将获得34%的折扣 通过亲核注射获得转基因小鼠的订单， 对通过胚泡注射产生基因靶向小鼠的影响。

The University of Michigan Multipurpose Arthritis Center Transgenic Animal Model Core provides genetically engineered mice to UM-UM-MAC investigators. Both conventional transgenic mice and mice with mutations induced by gene targeting in embryonic stem (ES) cells are produced for investigators. The Core guarantees that at least three transgenic mice will be produced for each transgene construct submitted. Core personnel provide quality tested reagents and work extensively with investigators to ensure the success for gene targeting experiments. The Core has an excellent track record. In the first four years of the preceding grant period, 687 transgenic mice were produced from 72 DNA constructs from 72 DNA constructs for 10 UM-MAC investigators. Mice with induced mutations in 12 genes were produced for 6 investigators. It is significant to note that 12 of the 13 gene targeted mice generated at the University of Michigan have been produced in the laboratories of UM-MAC investigators. Research of these animals by UM-MAC investigators has resulted in numerous publications in the fields of arthritis, musculoskeletal disease, inflammation, and gene regulation. We project that in the four years covered by the current proposal UM-MAC investigators will generate at least 80 requests for transgenic mice and 48 requests for production of germline ES cell-mouse chimeras for the generation of mouse strains with novel mutations. Dr. Sally Camper has used transgenic mice in her research since 1986 and is widely published in this field. Dr. Linda Samuelson established the technology of gene targeting in ES cells at the University of Michigan in 1990. Drs. Camper and Samuelson will work with the transgenic Core Steering Committee (composed of experienced users and a member of the UM- MAC Executive Committee) to monitor the Transgenic Core. Funding will be used to ensure that highly skilled personnel are available to meet the needs of UM-MAC investigators. Investigators will receive a 34% discount on orders for transgenic mice by pro-nuclear injection and a 34% discount on the production of gene targeted mice by blastocyst injection.