Investigating the cardio protective potential of exosomes from different cellular sources

研究不同细胞来源的外泌体的心脏保护潜力

• 批准号: 1906354
• 金额: --
• 依托单位: University College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1906354
• 关键词: Investigating; cardio; protective; potential; exosomes

Ishaemia and reperfusion (IR) injury is a major contributor to cardiomyocyte cell death following acute myocardial infarction. New ways of protecting the heart from IR injury are sought. Exosomes are small lipid-membrane vesicles released by all cell types, which contain proteins, lipids and miRNA that can signal between cells. It has previously been shown that exosomes from blood plasma or from endothelial cells are cardioprotective. Pilot data from this laboratory has shown that exosomes from certain stem cells (but not all) are cardioprotective. However, according to several studies, exosomes from fibroblsts are not. It is unclear what determines the cardioprotective protential of exosomes from different cellular sources. The aim of this project is to isolate exosomes from different primary cells and cell line soucces, characterize them, and evaluate their ability to protect cardiomycocytes using an in vitro assay simulating IT injury. By understanding the mechanism of protection by exosomes, we hope to identify the optimal cellular source for potential future use of exosomes to protect the hearts of patients.

缺血再灌注（IR）损伤是急性心肌梗死后心肌细胞死亡的主要原因。保护心脏免受IR损伤的新方法正在寻找。外泌体是由所有细胞类型释放的小脂膜囊泡，其含有可以在细胞之间传递信号的蛋白质、脂质和miRNA。先前已经表明，来自血浆或来自内皮细胞的外来体是心脏保护性的。该实验室的初步数据表明，来自某些干细胞（但不是全部）的外泌体具有心脏保护作用。然而，根据几项研究，来自成纤维细胞的外泌体不是。目前还不清楚是什么决定了来自不同细胞来源的外泌体的心脏保护潜力。本项目的目的是从不同的原代细胞和细胞系来源中分离外泌体，对其进行表征，并使用模拟IT损伤的体外试验评估其保护心肌细胞的能力。通过了解外泌体的保护机制，我们希望确定未来潜在使用外泌体保护患者心脏的最佳细胞来源。

项目成果

Exosomes from neuronal stem cells may protect the heart from ischaemia/reperfusion injury via JAK1/2 and gp130.

• DOI: 10.1111/jcmm.16515
• 发表时间: 2021-05
• 期刊: Journal of cellular and molecular medicine
• 影响因子: 5.3
• 作者: Katsur M;He Z;Vinokur V;Corteling R;Yellon DM;Davidson SM
• 通讯作者: Davidson SM