Investigating the cardio protective potential of exosomes from different cellular sources

缺血再灌注（IR）损伤是急性心肌梗死后心肌细胞死亡的主要原因。寻求保护心脏免受IR损伤的新方法。外泌体是由所有细胞类型释放的小脂质膜囊泡，其中含有蛋白质、脂质和可以在细胞之间传递信号的miRNA。先前已经证明来自血浆或内皮细胞的外泌体具有心脏保护作用。该实验室的初步数据表明，来自某些干细胞（但不是全部）的外泌体具有心脏保护作用。然而，根据一些研究，来自成纤维细胞的外泌体不是。目前尚不清楚是什么决定了来自不同细胞来源的外泌体的心脏保护潜力。该项目的目的是从不同的原代细胞和细胞系来源中分离外泌体，表征它们，并使用模拟IT损伤的体外实验评估它们保护心肌细胞的能力。通过了解外泌体的保护机制，我们希望确定最佳的细胞来源，以便将来使用外泌体来保护患者的心脏。

Ishaemia and reperfusion (IR) injury is a major contributor to cardiomyocyte cell death following acute myocardial infarction. New ways of protecting the heart from IR injury are sought. Exosomes are small lipid-membrane vesicles released by all cell types, which contain proteins, lipids and miRNA that can signal between cells. It has previously been shown that exosomes from blood plasma or from endothelial cells are cardioprotective. Pilot data from this laboratory has shown that exosomes from certain stem cells (but not all) are cardioprotective. However, according to several studies, exosomes from fibroblsts are not. It is unclear what determines the cardioprotective protential of exosomes from different cellular sources. The aim of this project is to isolate exosomes from different primary cells and cell line soucces, characterize them, and evaluate their ability to protect cardiomycocytes using an in vitro assay simulating IT injury. By understanding the mechanism of protection by exosomes, we hope to identify the optimal cellular source for potential future use of exosomes to protect the hearts of patients.