Core--Pathology

病理学核心负责对注射了泰勒氏病毒或用髓磷脂抗原免疫的各种小鼠品系进行所有形态学评估，以发展实验性自身免疫性脑脊髓炎。病理中心位于罗德里格斯博士的研究实验室。病理学核心的主要焦点主要是塑料嵌入的部分，无论是嵌入甲基丙烯酸乙二醇酯或阿拉迪特。这些是评估脱髓鞘、再髓鞘、脊髓萎缩和轴突丧失的标准检测方法。此外，脑和脊髓切片包埋在石蜡免疫细胞化学和原位杂交检测病毒复制。冷冻切片用于神经丝蛋白的免疫荧光染色。T细胞和细胞因子表达和[3H]石蜡毒素标记评估轴突完整性。

The Pathology Core is responsible for all the morphological assessments of various strains of mice injected with Theiler's virus, or immunized with myelin antigens for development of experimental autoimmune encephalomyelitis. The Pathology Core is housed in Dr. Rodriguez's research laboratory. The major focus of the Pathology Core is primarily on plastic-embedded sections embedded in either glycol methacrylate or Araldite. These are the standard assays for assessment of demyelination, remyelination, spinal cord atrophy, and axonal loss. In addition, brain and spinal cord sections are embedded in paraffin for immunocytochemistry and in situ hybridization to detect virus replication. Frozen sections are used for immunofluorescence staining for neurofilament proteins. T cell and cytokine expression and [3H] saxitoxin-labeling to assess axonal integrity.