Core--Pathology

核心--病理学

• 批准号: 6481262
• 负责人: MOSES RODRIGUEZ
• 金额: $ 19.52万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6481262
• 关键词: biomedical facility; histopathology; neuropathology

The Pathology Core is responsible for all the morphological assessments of various strains of mice injected with Theiler's virus, or immunized with myelin antigens for development of experimental autoimmune encephalomyelitis. The Pathology Core is housed in Dr. Rodriguez's research laboratory. The major focus of the Pathology Core is primarily on plastic-embedded sections embedded in either glycol methacrylate or Araldite. These are the standard assays for assessment of demyelination, remyelination, spinal cord atrophy, and axonal loss. In addition, brain and spinal cord sections are embedded in paraffin for immunocytochemistry and in situ hybridization to detect virus replication. Frozen sections are used for immunofluorescence staining for neurofilament proteins. T cell and cytokine expression and [3H] saxitoxin-labeling to assess axonal integrity.

病理核心负责对注射了泰勒氏病毒或用髓鞘抗原免疫的各种品系的小鼠进行所有形态评估，以发展实验性自身免疫性脑脊髓炎。病理学核心设在罗德里格斯博士的研究实验室里。病理核心的主要焦点主要是嵌入在乙二醇甲基丙烯酸酯或钠长石中的塑料包埋切片。这些是评估脱髓鞘、重新髓鞘形成、脊髓萎缩和轴突丢失的标准方法。此外，大脑和脊髓切片被包埋在石蜡中，用于免疫细胞化学和原位杂交，以检测病毒复制。冰冻切片用于神经丝蛋白的免疫荧光染色。T细胞和细胞因子表达及[~3H]肉毒杆菌毒素标记评估轴突完整性。