Core--Patient definition, 13C studies, postoperative tissue characterization

核心——患者定义、13C研究、术后组织表征

• 批准号: 6495441
• 负责人: DENNIS D SPENCER
• 金额: $ 22.55万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6495441
• 关键词: bioimaging /biomedical imaging; biomedical facility; brain imaging /visualization /scanning; cell population study; clinical research; data management; digital imaging; histology; human subject; partial seizure; stable isotope; temporal lobe /cortex disorder

The Core provides resources that are either directly shared by two or more projects or that promote project interaction. The PI is responsible for the Core and for assessing how each project interacts with the others. Glutamate clearance and energy metabolism derangement must be put into the context of patient selection, conduct of operative intervention, uniform description of tissue to be studied, and outcome regarding seizure control. The core will, therefore, be divided into four sections: Section 1: Preoperative definition of the patient population and presumed epileptogenic substrate. In this section, the PI will first define the patients that will undergo study, will obtain informed consent, will place electrodes in those patients requiring invasive study, and will make sure that all preoperative and qualitative and quantitative imaging and electrophysiology has been carried out (patient selection at conference, MRI, volumetrics, AVEEG, PET, MRS). Microdialysis assessment of glutamate will be provided both in vivo, when patients undergo invasive study, and in the slice. This data will be used correlatively in all the projects. Section: Intraoperative 13 study and tissue distribution. This involves digitized imaging of the brain intraoperatively to document the site of electrophysiological abnormalities and hippocampus in photographed, sectioned and numbered for investigator review. Section 3: Postoperative tissue characterization. This section is devoted to the neuronal and glial cell counts performed by Dr. Jung Kim which provides the cellular substrate for the metabolic and energy correlation studies. Section 4: Project coordination. The PI will provide coordination and data sharing among the projects assisted by an administrative associate who will maintain weekly conferences, coordinate patient movement among all the preoperative tests including the trips to Brookhaven National Laboratories. The administrator is also responsible for collecting and collating all data, coordinating interdisciplinary studies, and scheduling internal and external advisory meetings.

核心提供的资源要么直接由两个或多个项目共享，要么促进项目互动。PI负责核心，并评估每个项目如何与其他项目相互作用。谷氨酸清除和能量代谢紊乱必须纳入患者选择、手术干预的实施、待研究组织的统一描述以及癫痫控制的结果。因此，核心将分为四个部分：第1部分：患者人群的术前定义和假定的致癫痫基质。在本节中，PI将首先定义将接受研究的患者，将获得知情同意书，将在需要侵入性研究的患者中放置电极，并将确保已进行所有术前、定性和定量成像以及电生理学检查（在会议上选择患者、MRI、容积、AVEEG、PET、MRS）。当患者进行侵入性研究时，将在体内和切片中提供谷氨酸盐的微透析评估。这些数据将在所有项目中相关使用。章节：术中13研究和组织分布。这涉及术中脑的数字化成像，以记录电生理异常和海马的部位，并拍照、切片和编号以供研究者审查。第3节：术后组织表征。本节专门介绍Jung Kim博士进行的神经元和神经胶质细胞计数，为代谢和能量相关性研究提供细胞基质。第4节：项目协调。PI将在行政助理的协助下在项目之间进行协调和数据共享，行政助理将维持每周会议，协调所有术前检查之间的患者移动，包括前往布鲁克海文国家实验室。管理员还负责收集和整理所有数据，协调跨学科研究，并安排内部和外部咨询会议。