Apoptosis of ESNLCs

ESNLC的凋亡

• 批准号: 6410680
• 负责人: EUGENE M JOHNSON
• 金额: $ 17.53万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-12-01 至 2001-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6410680
• 关键词: apoptosis; cell line; developmental neurobiology; embryonic stem cell; laboratory mouse; nervous system regeneration; spinal cord injury; stem cell transplantation; terminal nick end labeling

Description: (from the abstract) Apoptotic cell death occurs in the nervous system during normal and in pathological situations. The PI's preliminary data indicate that apoptosis has a major role in the ES cell graft after transplant into the injured rodent spinal cord. The extent of cell death underlie this process are currently unknown. The goal of this proposal is to apoptotic cell death of ES cell-derived neural lineage cells (ESNLCs). In relevant stages of development. In the second, he will characterize the apoptosis-inducing activity of the syrinx fluid which accumulates in the spinal apoptosis-inducing activity, and determine the changes in this activity as a function of time post-injury. Third, he will utilize the paradigms developed in to the molecular mechanisms of apoptosis and to develop cell clines that are resistant to apoptosis. These experiments should help to define the optimal molecules that are responsible for apoptosis induction seen after the success of ESNLC transplants.

描述：（来自摘要）凋亡细胞死亡发生在神经系统在正常和病理情况下。PI的初步数据表明，细胞凋亡在移植到受损的啮齿动物脊髓后的ES细胞移植物中起主要作用。细胞死亡的程度在这个过程中是目前未知的。本研究的目的是探讨胚胎干细胞来源的神经细胞系细胞（ESNLC）的凋亡。在相关的发展阶段。在第二部分中，他将描述在脊髓强直诱导活动中积累的空洞液的强直诱导活动，并确定该活动随损伤后时间的变化。第三，他将利用细胞凋亡的分子机制和发展细胞株是抗凋亡的范例。这些实验应该有助于确定ESNLC移植成功后负责细胞凋亡诱导的最佳分子。