Apoptosis of ESNLCs

ESNLC的凋亡

• 批准号: 6565293
• 负责人: EUGENE M JOHNSON
• 金额: $ 17.53万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6565293
• 关键词: apoptosis; cell line; developmental neurobiology; embryonic stem cell; laboratory mouse; nervous system regeneration; spinal cord injury; stem cell transplantation; terminal nick end labeling

Description: (from the abstract) Apoptotic cell death occurs in the nervous system during normal and in pathological situations. The PI's preliminary data indicate that apoptosis has a major role in the ES cell graft after transplant into the injured rodent spinal cord. The extent of cell death underlie this process are currently unknown. The goal of this proposal is to apoptotic cell death of ES cell-derived neural lineage cells (ESNLCs). In relevant stages of development. In the second, he will characterize the apoptosis-inducing activity of the syrinx fluid which accumulates in the spinal apoptosis-inducing activity, and determine the changes in this activity as a function of time post-injury. Third, he will utilize the paradigms developed in to the molecular mechanisms of apoptosis and to develop cell clines that are resistant to apoptosis. These experiments should help to define the optimal molecules that are responsible for apoptosis induction seen after the success of ESNLC transplants.

描述：(摘自摘要)在正常和病理情况下，神经系统都会发生细胞凋亡性死亡。PI的初步数据表明，在ES细胞移植到受损的啮齿动物脊髓中后，细胞凋亡在移植中起主要作用。这一过程背后的细胞死亡程度目前尚不清楚。这项建议的目的是减少ES细胞衍生的神经系细胞(ESNLCs)的细胞死亡。处于相关的发展阶段。在第二个实验中，他将表征脊髓空洞液中积聚的细胞凋亡诱导活性，并确定这一活性在损伤后随时间的变化。第三，他将利用所开发的范例来研究细胞凋亡的分子机制，并开发对细胞凋亡具有抵抗力的细胞克隆。这些实验应该有助于确定在ESNLC移植成功后，负责诱导细胞凋亡的最佳分子。