DEVELOPMENT OF 3D DIGITAL HOLOGRAPHY FOR TRACKING MOSQUITO FLIGHT 1=Engineering 2=Sensors and Instrumentation

在大体积、房间大小的空间上进行定量、高动态范围的3D成像存在根本性的挑战。这个项目的动机是我们的团队在量化蚊子行为方面的工作，以便设计新的干预措施来减轻传染病的传播，这是与利物浦热带医学院（LSTM）合作的。据我们所知，我们是目前唯一一个在如此全面和详细的水平上研究蚊子在体内空间活动的小组。UoW光学工程师和LSTM媒介生物学家之间的合作始于2011年，并开发了独特的视频跟踪系统，能够前所未有地表征经杀虫剂处理的蚊帐中按蚊和库蚊的寻宿主行为。我们的研究方法是独特的，允许调查以前不可能的媒介行为，并且已经被证明是一种快速和富有成效的新工具。这两家集团共有7个项目奖（目前有5个）。目前的活动主要是应用研究，涉及光学成像和数据分析，以获得自然行为。该博士项目提案旨在研究一种基于数字全息（DH）的更具挑战性的3D成像方法，以克服现有方法的局限性：获得的3D位置信息的准确性，在现场实施的便利性，在扩展体积上进行测量的能力以及通过蚊帐材料成像的能力。DH是一种相对较新的成像方式，其中从场景散射的复杂波前在数字探测器上进行干涉记录。采用计算方法将波前反向传播到测量体积内的感兴趣平面，从而实现相位和振幅的重建。大多数成功的DH应用都在微米到毫米的尺度上。这个项目将探索在微米级分辨率下，将DH的动态范围扩展到米级体积。检测散射场相位的能力预计将特别有助于对蚊子进行成像，这将产生特征相位扰动，可用于指导重建算法以识别单个蚊子的平面，从而识别3D位置。研究成果将在《光学快报》、《光学快报》、《应用光学》等光学工程期刊上发表。LSTM已经证实，他们很高兴支持在他们的昆虫饲养场中对该仪器进行试验，例如检查蚊子接近蚊帐缺陷的详细行为和新型捕蚊器的设计。因此，可以期望在更高排名的期刊上发表更多的文章。我们现有的项目将为该领域的适当开发提供一个平台。

There are fundamental challenges to quantitative, high dynamic range 3D imaging over large volumes, room sized spaces. This project is motivated by our groups work in quantifying mosquito behaviour in order to design novel and new interventions to mitigate the spread of infectious disease which is a collaboration with Liverpool School of Tropical Medicine (LSTM). To our knowledge, we are the only group currently investigating in vivo spatial activity of mosquitoes at such a comprehensive and detailed level. The collaboration between UoW optical engineers and LSTM vector biologists started in 2011 and has developed unique video tracking systems that have enabled unprecedented characterisation of Anopheles sp. and Culex sp. mosquito host-seeking behaviour at insecticide treated bed nets. Our research approach is distinctive, permitting investigation of vector behaviours not previously possible, and already shown to be a rapid and productive route to new tools. The groups have a joint track record of 7 project awards (5 current). The current activities are largely applied research involving optical imaging and data analytics to derive natural behaviours. This PhD project proposal seeks to investigate a more challenging 3D imaging methodology based on digital holography (DH) to overcome the limitations of existing approaches: accuracy of the 3D positional information obtained, ease of implementation in the field, ability to measure over an extended volume and to image through bednet materials. DH is a relatively new imaging modality in which the complex wavefront scattered from a scene is recorded interferometrically at a digital detector. Computational methods are employed to back propagate the wavefront to planes of interest within the measurement volume enabling reconstructions of both phase and amplitude. The majority of successful DH applications have been at the micron to millimetre scale. This project will explore extending the dynamic range of DH to metre scale volumes within micron scale resolution. The ability to detect the phase of the scattered field is expected to be particularly helpful in imaging mosquitoes which will generate a characteristic phase perturbation which can be used to guide the reconstruction algorithms to identify the plane, and hence 3D position, of individual mosquitoes. The outputs of the research will be reported in high ranking optical engineering journals, such as Optics Express, Optics Letters and Applied Optics. LSTM have confirmed that they are happy to support trials of the instrument in their insectaries to examine, for example, detailed behaviour of mosquitoes approaching faults in bednets and the design of novel mosquito traps. Hence, further publications can be expected in higher ranking journals. Our existing projects will provide a platform for exploitation in the field as appropriate.