DEVELOPMENT OF 3D DIGITAL HOLOGRAPHY FOR TRACKING MOSQUITO FLIGHT 1=Engineering 2=Sensors and Instrumentation

在大体积、房间大小的空间上进行定量、高动态范围3D成像存在根本挑战。该项目的动机是我们的团队量化蚊子行为的工作，以设计新颖的干预措施来减轻传染病的传播，这是与利物浦热带医学院（LSTM）的合作。据我们所知，我们是目前唯一一个在如此全面和详细的水平上研究蚊子体内空间活动的小组。UoW光学工程师和LSTM载体生物学家之间的合作始于2011年，并开发了独特的视频跟踪系统，使按蚊和库蚊在杀虫剂处理过的蚊帐中寻找宿主的行为具有前所未有的特征。我们的研究方法是独特的，允许以前不可能的媒介行为的调查，并已被证明是一个快速和富有成效的新工具的路线。这些团体有7个项目奖（目前有5个）的共同记录。目前的活动主要是应用研究，涉及光学成像和数据分析，以得出自然行为。该博士项目提案旨在研究一种基于数字全息术（DH）的更具挑战性的3D成像方法，以克服现有方法的局限性：获得的3D位置信息的准确性，在现场实施的方便性，在扩展体积上测量的能力以及通过蚊帐材料成像的能力。DH是一种相对较新的成像方式，其中从场景散射的复杂波前在数字检测器处被干涉地记录。采用计算方法将波前反向传播到测量体积内的感兴趣平面，从而能够重建相位和振幅。大多数成功的DH应用都是在微米到毫米级。该项目将探索将DH的动态范围扩展到微米级分辨率内的米级体积。检测散射场的相位的能力预期在对蚊子进行成像时特别有帮助，这将生成特征相位扰动，该特征相位扰动可用于引导重建算法以识别个体蚊子的平面，并因此识别个体蚊子的3D位置。研究成果将在高级光学工程期刊上发表，如Optics Express，Optics Letters和Applied Optics。LSTM已经证实，他们很乐意支持在他们的昆虫实验室中进行该仪器的试验，例如，研究蚊子接近蚊帐故障的详细行为以及新型捕蚊器的设计。因此，可以预期在更高级别的期刊上发表更多的文章。我们现有的项目将提供一个适当的开发平台。

There are fundamental challenges to quantitative, high dynamic range 3D imaging over large volumes, room sized spaces. This project is motivated by our groups work in quantifying mosquito behaviour in order to design novel and new interventions to mitigate the spread of infectious disease which is a collaboration with Liverpool School of Tropical Medicine (LSTM). To our knowledge, we are the only group currently investigating in vivo spatial activity of mosquitoes at such a comprehensive and detailed level. The collaboration between UoW optical engineers and LSTM vector biologists started in 2011 and has developed unique video tracking systems that have enabled unprecedented characterisation of Anopheles sp. and Culex sp. mosquito host-seeking behaviour at insecticide treated bed nets. Our research approach is distinctive, permitting investigation of vector behaviours not previously possible, and already shown to be a rapid and productive route to new tools. The groups have a joint track record of 7 project awards (5 current). The current activities are largely applied research involving optical imaging and data analytics to derive natural behaviours. This PhD project proposal seeks to investigate a more challenging 3D imaging methodology based on digital holography (DH) to overcome the limitations of existing approaches: accuracy of the 3D positional information obtained, ease of implementation in the field, ability to measure over an extended volume and to image through bednet materials. DH is a relatively new imaging modality in which the complex wavefront scattered from a scene is recorded interferometrically at a digital detector. Computational methods are employed to back propagate the wavefront to planes of interest within the measurement volume enabling reconstructions of both phase and amplitude. The majority of successful DH applications have been at the micron to millimetre scale. This project will explore extending the dynamic range of DH to metre scale volumes within micron scale resolution. The ability to detect the phase of the scattered field is expected to be particularly helpful in imaging mosquitoes which will generate a characteristic phase perturbation which can be used to guide the reconstruction algorithms to identify the plane, and hence 3D position, of individual mosquitoes. The outputs of the research will be reported in high ranking optical engineering journals, such as Optics Express, Optics Letters and Applied Optics. LSTM have confirmed that they are happy to support trials of the instrument in their insectaries to examine, for example, detailed behaviour of mosquitoes approaching faults in bednets and the design of novel mosquito traps. Hence, further publications can be expected in higher ranking journals. Our existing projects will provide a platform for exploitation in the field as appropriate.