Making vitamin H sustainable - developing biotin overproduction approaches

使维生素 H 可持续 - 开发生物素过量生产方法

• 批准号: 1922883
• 金额: --
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-1922883
• 关键词: Making; vitamin; sustainable; developing; biotin

My research aims to create a functional and improved BioB enzyme to overall improve the production of biotin. My objectives are to create variants of BioB enzyme that have a faster turnover rate than existing E. coli BioB and allow us to overcome the first kinetic bottleneck in the biotin biosynthesis pathway. The ways in which I hope to achieve this is through rational design create mutants of this enzyme in E. coli. This will enable me confirm the range of impact that may be accessible in the natural enzymes and the role of these residues on the reactivity.

我的研究旨在创造一种功能性和改进的BioB酶，以全面提高生物素的生产。我的目标是创造比现有的E. coliBioB中，使我们能够克服生物素生物合成途径中的第一个动力学瓶颈。我希望通过合理的设计，在大肠杆菌中创造这种酶的突变体。杆菌这将使我能够确认天然酶中可能存在的影响范围以及这些残留物对反应性的作用。