REDUCED LORDOSIS BEHAVIOR AFTER INTRACEREBRAL 8-OH-DPAT

脑内 8-OH-DPAT 后可减少脊柱前凸行为

• 批准号: 6520893
• 负责人: LYNDA L UPHOUSE
• 金额: $ 21.8万
• 依托单位: TEXAS WOMAN'S UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 2004-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6520893
• 关键词: GABA aminotransferase; behavioral /social science research tag; brain mapping; cyclic AMP; electron microscopy; estrus; female; hormone regulation /control mechanism; hypothalamus; immunocytochemistry; in situ hybridization; laboratory rat; neurochemistry; norepinephrine; protein kinase C; receptor coupling; receptor sensitivity; serotonin; serotonin inhibitor; serotonin receptor; sex behavior

DESCRIPTION (Adapted From The Applicant's Abstract): The Proposed studies are designed to provide information about the mechanisms and physiological significance of interactions among serotonin (5-HT) receptors in the control of female rat lordosis behavior. Emphasis is placed on 5-HT's role in the mediobasal hypothalamus (MBH), especially the ventromedial nucleus of the hypothalamus (VMN). The specific aims of the present application are to identify mechanisms responsible for this interaction. Studies are also designed to test the hypothesis that 5-HT's dual function within the VMN enables the organism to modulate its behavioral repertoire in a manner conducive to both species and individual survival. The specific aims of the proposed studies are: To determine the relative distribution of 5-HT1A, 5-HT2A and 5-HT2C receptors within the VMN of proestrous rats and of hormonally-primed, ovariectomized rats. To test intracellular mechanisms responsible for the effects of 5-HT1A and 5-HT2A/2C receptors. Experiments are proposed to test the hypothesis that 5-HT1A receptor-mediated inhibition of cAMP accumulation is involved in the receptor's inhibitory effect on lordosis behavior and that inhibition of cAMP accumulation is involved in the receptor's inibitory effect on lordosis behavior and that 5-HT2 receptors attenuate this inhibition through actions involving protein kinase C. Experiments are proposed to test the hypothesis that 5-HT1A receptors reduce the release of norepinephrine (NE) and that 5-HT2 receptors enhance the release of gamma-aminobutyric acid (GABA); and that these effects of 5-HT on NE and/or GABA contribute to 5-HT's ability to both inhibit and facilitate lordosis behavior. To test a model about the physiological significance of 5-HT's dual role in the control of lordosis behavior. Experiments are designed to evaluate the hypothesis that 5-HT2 receptors reduce inhibition of lordosis behavior (mediated via 5-HT1A receptors) that is initiated by environmental challenge.

描述（改编自申请人的摘要）： 拟议的研究旨在提供有关机制的信息 5-羟色胺（5-HT）受体之间相互作用的生理意义 在控制雌性大鼠脊柱前凸行为方面。重点放在5-羟色胺上 在下丘脑内侧基底核（MBH），尤其是腹内侧核中的作用 下丘脑（VMN）。本申请的具体目的是 确定负责这种相互作用的机制。研究还设计了 为了检验5-HT在VMN中的双重功能使VMN成为可能的假设， 生物体以有利于两者的方式调节其行为库 物种和个体生存 拟议研究的具体目标是： 确定5-HT 1A、5-HT 2A和5-HT 2C受体的相对分布 在动情前期大鼠和经尿道致敏的卵巢切除大鼠的VMN中， 大鼠 为了测试负责5-HT 1A和 5-HT 2A/2C受体。提出实验来检验这一假设， 5-HT 1A受体介导的cAMP积累抑制参与了 受体对脊柱前凸行为抑制作用及cAMP的抑制作用 积累参与受体对脊柱前凸的抑制作用 5-HT 2受体通过作用于神经元的神经元， 涉及蛋白激酶C。 提出实验来检验5-HT 1A受体减少 去甲肾上腺素（NE）和5-HT 2受体释放促进释放 γ-氨基丁酸（GABA）; 5-HT对NE和/或 GABA有助于5-HT抑制和促进脊柱前凸的能力 行为 为了验证5-HT在脑缺血中的双重作用的生理意义， 脊柱前凸行为的控制。设计实验来评估 5-HT 2受体减少脊柱前凸行为抑制的假说 （通过5-HT 1A受体介导）由环境挑战引发。