PERIVASCULAR BRAIN MACROPHAGES ARE MAJOR TARGET OF SIV INFECTION

不同的单核/巨噬细胞群的鉴定 将病毒携带到CNS以及CNS内的子集 艾滋病毒/SIV感染的目标在这项研究中没有定义 免疫组织化学及免疫组织化学与IN联合应用 用原位杂交法确定脑巨噬细胞的哪些群体 在病毒血症高峰期和SIVE血管周围脑内感染 巨噬细胞和常驻的脑巨噬细胞，小胶质细胞，都是 CD11b和CD68在血管周围巨噬细胞呈阳性，但不呈阳性 小胶质细胞也表达CD14、CD45和CD86多核 巨细胞(MNGC)如CD11b和CD68阳性 实质小胶质细胞和血管周围巨噬细胞也是 CD14和CD45阳性，但表达可变到不可检测的水平 免疫组织化学和原位杂交相结合的方法检测CD4 和/或髓系细胞标记物的双标记免疫组织化学 其次是gp120，我们演示了gp120或 病毒RNA阳性细胞是CD14阳性的血管周围巨噬细胞 这些血管周围细胞是中枢神经系统内感染的主要细胞。 在病毒血症高峰期(感染后2周)和SIVE动物中 不到1%的受感染细胞是常驻的小胶质细胞，基于 免疫组织化学这些数据强调了 血管周围巨噬细胞是SIV在中枢神经系统的主要靶点

The identification of distinct monocyte/macrophage populations that carry virus to the CNS and subsets within the CNS that are the target for infection by HIV/SIV is not defined In this study we have used immunohistochemistry and combined immunohistochemistry with in situ hybridization to define which populations of brain macrophages are infected at peak viremia and in SIVE Perivascular brain macrophages and the resident brain macrophage, microglia, are both CDllb and CD68 positive The perivascular macrophages, but not microglia, are also positive for CD14, CD45, and CD86 Multi-nucleated giant cells (MNGC), when present, are CD11b and CD68 positive like parenchymal microglia and perivascular macrophages The MNGC also are CD14 and CD45 positive, but express variable to non-detectable levels of CD4 Using combined immunohistochemistry and in situ hybridization and/or double label immunohistochemistry for myeloid cell markers followed by gp120, we demonstrat e th at the majority of gp120 or viral RNA positive cells are CD14 positive perivascular macrophages These perivascular cells are the major cell infected within the CNS during peak viremia (2 weeks post infection) and in animals with SIVE Less than 1% of the infected cells are resident microglia based upon immunohistochemistry These data underscore the importance of the perivascular macrophage as the major target of SIV in the CNS