CHOLESTEROL CRYSTAL FORMATION IN REAL & MODEL BIOMEMBRANES

真实的胆固醇晶体形成

• 批准号: 6491117
• 负责人: GERALD W FEIGENSON
• 金额: $ 14.27万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-15 至 2002-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6491117
• 关键词: bioimaging /biomedical imaging; biological products; biomedical resource; proteins; radiography; structural biology

During two beam-time accesses at CHESS, each of 48 hours duration, we conducted feasibility studies with regard to the prospect of data collection on crystals of several complexes of type 1 human 17b-Hydroxysteroid Dehydrogenase (17b-HSD1) and small crystals of other steroidogenic enzymes, such as human Cytochrome P450 aromatase. We were able to record diffraction under cryogenic conditions and establish the feasibility of data collection on several of these crystals. Two partial data sets were collected on the crystals of equilin- and coumestrol-complexes of 17b-HSD1. Despite being small (< 0.2mm) and thin (< 0.05mm), both these crystals diffracted to about 2.5
resolution. Data sets were about 55-75% complete. More beam time is required to complete the data collection. Our regular application for more beam time has recently received a favorable rating, permitting us to continue with the process. Preliminary structure analyses with these data sets have enabled us to locate equilin and coumestrol molecules in the active site of 17b-HSD1. These results are being presented at the upcoming Endocrine Society meeting in New Orleans.

在CHESS的两次射束时间访问期间，每次持续48小时， 我们对数据的前景进行了可行性研究 收集1型人的几种复合物的晶体 17 b-羟基类固醇脱氢酶（17 b-HSD 1）和小晶体 其它类固醇生成酶，如人细胞色素P450芳香酶。 我们能够在低温条件下记录衍射， 确定就其中几个问题收集数据的可行性 晶体 收集了两个部分数据集的晶体， 17 b-HSD 1的马烯雌酚和香豆雌酚复合物。 虽然很小（ 0.2mm）和薄（< 0.05mm），这两种晶体衍射到约 2.5
分辨率 数据集大约完成了55-75%。 多个波束 需要时间来完成数据收集。 我们的常规 申请更多的光束时间最近收到了有利的 评级，允许我们继续这个过程。 初步 对这些数据集的结构分析使我们能够找到 马烯雌酚和香豆雌酚分子在17 b-HSD 1的活性位点。 这些结果将在即将召开的内分泌学会 在新奥尔良见面。