CHOLESTEROL CRYSTAL FORMATION IN REAL & MODEL BIOMEMBRANES

在国际象棋的两次波束时间访问期间，每次持续48小时， 我们就数据的前景进行了可行性研究 几种人1型络合物的晶体收集 17b-羟基类固醇脱氢酶(17b-HSD1)及其小晶体 其他类固醇生成酶，如人细胞色素P450芳香酶。 我们能够在低温条件下记录衍射， 确定对其中几个项目收集数据的可行性 水晶。收集了两个关于晶体的部分数据集 17b-HSD1的马钱子素和香豆素络合物。尽管很小(&lt； 0.2 mm)和薄(&lt；0.05 mm)，这两种晶体的衍射率都约为 2.5决议。数据集大约完成了55%-75%。更多光束 完成数据收集需要时间。我们的常客 申请更多的光束时间最近收到了有利的结果 评级，允许我们继续进行这一过程。初步 对这些数据集的结构分析使我们能够定位 17b-HSD1活性部位的马琳和香豆素分子。 这些结果将在即将到来的内分泌学会上公布 在新奥尔良会面。

During two beam-time accesses at CHESS, each of 48 hours duration, we conducted feasibility studies with regard to the prospect of data collection on crystals of several complexes of type 1 human 17b-Hydroxysteroid Dehydrogenase (17b-HSD1) and small crystals of other steroidogenic enzymes, such as human Cytochrome P450 aromatase. We were able to record diffraction under cryogenic conditions and establish the feasibility of data collection on several of these crystals. Two partial data sets were collected on the crystals of equilin- and coumestrol-complexes of 17b-HSD1. Despite being small (< 0.2mm) and thin (< 0.05mm), both these crystals diffracted to about 2.5
resolution. Data sets were about 55-75% complete. More beam time is required to complete the data collection. Our regular application for more beam time has recently received a favorable rating, permitting us to continue with the process. Preliminary structure analyses with these data sets have enabled us to locate equilin and coumestrol molecules in the active site of 17b-HSD1. These results are being presented at the upcoming Endocrine Society meeting in New Orleans.