MACCHESS CONSORTIUM FOR SYNCHROTRON RADIATION IN MOLEC MED: TCR, MHC

MOLEC MED 同步辐射的 MACCHESS 联盟：TCR、MHC

• 批准号: 6491154
• 负责人: DON C WILEY
• 金额: $ 14.27万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-15 至 2002-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6491154
• 关键词: bioimaging /biomedical imaging; biological products; biomedical resource; proteins; radiography; structural biology

We have now been able to freeze all the virus crystals mentioned below. Thus, nearly all our data this year were of frozen crystals. This created vast new possibilities. In the past, we have had to use one new crystal every two to six exposures (depending on the crystals). Now we can collect a whole data set (often almost 500 exposures) with a single crystal. That makes possible a vast new array of experiments with mutants and ligands that were not readily possible previously. (a) Parvoviruses The following conditions were explored for feline and canine parvoviruses. (i) pH changes from 5.5 to 7.2. This alters the ability to hemagglutinate, in other words, alters the ability of the virus to bind to specific receptors in the Rhesus monkey erythrocytes. (ii) The effect of Ca2+ ions. These ions are associated with changes of conformation of the hemagglutinating loop on the viral surface. (iii) Soaking and co-crystallization with various sialic acids, which are the terminal carbohydrate residues used by the virus in binding host cells. We have also obtained some initial diffraction patterns of porcine parvovirus capsids assembled in a baculovirus expression system. (b) Picornaviruses We have collected complete data sets of some antiviral compounds bound to human rhinovirus 16 (HRV16). We have collected complete data sets of some drug-dependent HRV16 mutants. These viruses are unstable in the absence of drug. We collected some MAD data on ICAM-1, the receptor to the major group of rhinoviruses. We solved this structure and have fitted the structure into cryo-EM reconstructions of the complex of ICAM-1 with HRV16. This is the first atomic resolution study of a proteinaceous receptor interaction with a virus. We obtained some initial results on crystals of encephalomyocarditis virus. (c) Microviridae We have collected a partial, relatively low resolution data set of a3, an E. coli bacteriophage homologous to fX174. (d) Other Results We collected data on crystals of a mutant of the "whisker" protein of phage T4 (this was "warm" data). We obtained initial results of crystals of the prolate head of phage f2

我们现在已经能够冻结所有提到的病毒晶体 下面 因此，我们今年几乎所有的数据都是冷冻晶体。 这创造了巨大的新的可能性。 在过去，我们不得不使用 每两到六次曝光一个新的晶体（取决于 晶体）。 现在我们可以收集整个数据集（通常接近500个 曝光）与单晶。 这使得一个巨大的新的 一系列的突变体和配体实验， 可能以前。 (a)在以下条件下， 探索猫和犬细小病毒。 (i)pH值从5.5 到7.2。 这改变了血细胞凝集的能力，换句话说， 改变了病毒与细胞中特定受体结合的能力， 恒河猴红细胞。 (ii)Ca 2+离子的影响。 这些离子 与血凝蛋白构象的变化有关 病毒表面的环。 (iii)浸泡和共结晶， 各种唾液酸，它们是末端碳水化合物残基 病毒用来结合宿主细胞 我们还获得了一些 组装的猪细小病毒衣壳的初始衍射图 在杆状病毒表达系统中。 (b)小核糖核酸病毒 收集了一些抗病毒化合物的完整数据集 人鼻病毒16（HRV 16）。 我们收集了完整的数据集， 一些药物依赖性HRV 16突变体。 这些病毒在 没有药物。 我们收集了一些关于ICAM-1的MAD数据， 主要的鼻病毒群。 我们解决了这个结构， 将结构拟合到复合物的冷冻电镜重建中， ICAM-1与HRV 16。 这是第一个原子分辨率的研究， 蛋白质受体与病毒的相互作用。 我们得到了一些 脑心肌炎病毒晶体的初步结果。 （c）第（1）款 我们已经收集了一部分，相对较低的分辨率 a3的数据集，E.大肠杆菌噬菌体fX 174同源。 （d）其他事项 其他结果我们收集了一个突变体的晶体数据， 噬菌体T4的“须”蛋白（这是“暖”数据）。 我们获得 噬菌体f2扁长头晶体的初步结果