MACCHESS CONSORTIUM FOR SYNCHROTRON RADIATION IN MOLEC MED: TCR, MHC

MOLEC MED 同步辐射的 MACCHESS 联盟：TCR、MHC

• 批准号: 6667831
• 负责人: DON C WILEY
• 金额: $ 14.27万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2003-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6667831
• 关键词: bioimaging /biomedical imaging; biological products; biomedical resource; proteins; radiography; structural biology

We have now been able to freeze all the virus crystals mentioned below. Thus, nearly all our data this year were of frozen crystals. This created vast new possibilities. In the past, we have had to use one new crystal every two to six exposures (depending on the crystals). Now we can collect a whole data set (often almost 500 exposures) with a single crystal. That makes possible a vast new array of experiments with mutants and ligands that were not readily possible previously. (a) Parvoviruses The following conditions were explored for feline and canine parvoviruses. (i) pH changes from 5.5 to 7.2. This alters the ability to hemagglutinate, in other words, alters the ability of the virus to bind to specific receptors in the Rhesus monkey erythrocytes. (ii) The effect of Ca2+ ions. These ions are associated with changes of conformation of the hemagglutinating loop on the viral surface. (iii) Soaking and co-crystallization with various sialic acids, which are the terminal carbohydrate residues used by the virus in binding host cells. We have also obtained some initial diffraction patterns of porcine parvovirus capsids assembled in a baculovirus expression system. (b) Picornaviruses We have collected complete data sets of some antiviral compounds bound to human rhinovirus 16 (HRV16). We have collected complete data sets of some drug-dependent HRV16 mutants. These viruses are unstable in the absence of drug. We collected some MAD data on ICAM-1, the receptor to the major group of rhinoviruses. We solved this structure and have fitted the structure into cryo-EM reconstructions of the complex of ICAM-1 with HRV16. This is the first atomic resolution study of a proteinaceous receptor interaction with a virus. We obtained some initial results on crystals of encephalomyocarditis virus. (c) Microviridae We have collected a partial, relatively low resolution data set of a3, an E. coli bacteriophage homologous to fX174. (d) Other Results We collected data on crystals of a mutant of the "whisker" protein of phage T4 (this was "warm" data). We obtained initial results of crystals of the prolate head of phage f2

我们现在已经能够冻结所有提到的病毒晶体 下面。因此，我们今年几乎所有的数据都是冰冻晶体。 这创造了巨大的新可能性。在过去，我们不得不使用 每两到六次曝光就有一个新晶体(取决于 水晶)。现在我们可以收集整个数据集(通常接近500个 曝光)和单晶。这使得一个巨大的新的 一系列使用突变体和配体的实验，这些实验并不容易 以前有可能。(A)细小病毒符合下列条件 探索猫和狗的细小病毒。(I)酸碱值由5.5开始变化 到7.2。这改变了血凝的能力，换句话说， 改变病毒与体内特定受体结合的能力 恒河猴红细胞。(Ii)钙离子的影响。这些离子 与血凝素构象的变化有关 在病毒表面形成环状。(Iii)浸泡和共结晶 各种唾液酸，它们是碳水化合物的末端残基 被病毒用来结合宿主细胞。我们还获得了一些 猪细小病毒衣壳组装的初始衍射图 在杆状病毒表达系统中。(B)我们有小核糖核酸病毒 收集的一些抗病毒化合物的完整数据集与 人鼻病毒16型(HRV16)。我们已经收集了完整的数据集 一些药物依赖的HRV16突变体。这些病毒在人体内不稳定 没有吸毒。我们收集了一些有关ICAM-1受体的MAD数据 鼻病毒的主要群体。我们解决了这个结构，并且已经 将该结构用于冷冻-EM重建的 带有HRV16的ICAM-1。这是第一次对原子分辨率进行研究 蛋白质受体与病毒的相互作用。我们得到了一些 脑心肌炎病毒晶体的初步研究结果。(C) 小病毒科我们收集了一个部分的，相对较低的分辨率 与fX174同源的大肠杆菌噬菌体A3的数据集。(D) 其他结果我们收集了一种突变体的晶体数据 噬菌体T4的“晶须”蛋白(这是“热的”数据)。我们获得了 噬菌体f2长头部结晶的初步结果