DETERMINATION OF OLIGOMERIC STATE OF HSV GLYCOPROTEINS GD, GH & GL, USING STEM
DETERMINATION OF OLIGOMERIC STATE OF HSV GLYCOPROTEINS GD, GH & GL, USING STEM
批准号:
6444690
负责人:
GARY H COHEN
金额:
$29.31万
依托单位国家:
美国
项目类别:
财政年份:
2001
资助国家:
美国
项目状态:
已结题
起止时间:
2001-04-01 至 2002-09-29
中文摘要
糖蛋白D(GD)是一种重要的囊膜蛋白。
疱疹。单纯疱疹病毒(HSV)HSV-1和HSV-2都是
含有gD,且gD-1和gD-2在序列上为800/6同源。
这两种蛋白质的截短版本已经在一种
杆状病毒表达系统。这些蛋白质含有所有的氨基
如体外试验所示,功能所必需的酸具有
适当的构象,并且是可溶的。因为它们被截断为
在疏水跨膜区之前的氨基酸306。他们
分别命名为GD-1(306T)和GD-2(306T)。另外两个突变体
Gd-1(306T)基因已被克隆和表达。而野生的则有
三个N-连接的碳水化合物,GD-I(Taat)只有一个,GD I(QAAt)
什么都没有。这两种蛋白质都具有适当的构象和功能
体外试验。Gd-1(306t)蛋白凝胶呈二聚体
过滤数据。STEM中的质量分析与此一致,并且
对所有四个质点的额外测量表明,它们是
都是二聚体。在病毒中发现了单纯疱疹病毒糖蛋白H和L
以杂化齐聚物形式存在的包膜。纯化的Gh/Gl复合体正在进行
在STEM中进行分析以确定其分子量并查看是否
任何结构细节都可以观察到。Gl)(二聚体)/HSV受体
复合体也将使用STEM进行研究。
英文摘要
Glycoprotein D (gD) is an essential envelope protein from the
herpes. simplex virus (HSV). The strains HSV-1 and HSV-2 both
contain gD, and gD-1 and gD-2 are 800/6 homologous in sequence.
Truncated versions of the two proteins have been cloned in a
baculovirus expression system. These proteins have all the amino
acids necessary for function as shown by in vitro assays, have the
proper conformation, and are soluble. since they are truncated at
amino acid 306 prior to the hydrophobic transmembrane region. They
are called gD-1(306t) and gD-2(306t). Two additional mutants of
gD-1(306t) have.been cloned and expressed. Whereas the wild-type has
three N-linked carbohydrates, gD-I(TAAt) has only one and gD I (QAAt)
has none. Both proteins have the -proper conformation and function in
in vitro assays. The protein gD-1(306t) appears dimeric by gel
filtration data. Mass analysis in the STEM agrees with this and
additional measurements on all four of these protems indicate they are
all dimers. The HSV glycoproteins H and L are found in the virus
envelope as a hetero-oligomer. The purified gH/gL complex is being
analyzed in the STEM to determine its molecular weight and to see if
any structural details can be observed. The gl) (dimer)/HSV receptor
complex is also going to be studied using the STEM.
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批准号:8233374
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项目类别:
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资助金额:$42.31万
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财政年份:2011
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负责人:GARY H COHEN
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依托单位:
CORE--BACULOVIRUS
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批准号:6654640
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资助金额:$10.35万
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批准号:6562067
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资助金额:$23.78万
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财政年份:2002
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负责人:GARY H COHEN
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CORE--BACULOVIRUS
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批准号:6481257
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资助金额:$10.35万
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财政年份:2001
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负责人:GARY H COHEN
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CORE--BACULOVIRUS
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批准号:6324783
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项目类别:
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资助金额:$16.89万
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财政年份:2000
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负责人:GARY H COHEN
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依托单位:
DETERMINATION OF OLIGOMERIC STATE OF HSV GLYCOPROTEINS GD, GH & GL, USING STEM
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批准号:6308938
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项目类别:
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资助金额:$0.97万
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财政年份:2000
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负责人:GARY H COHEN
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依托单位:
CORE--BACULOVIRUS
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批准号:6112417
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项目类别:
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资助金额:$16.89万
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财政年份:1999
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负责人:GARY H COHEN
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依托单位:
FUNCTIONAL ANALYSIS OF GLYCOPROTEIN GD OF HERPES SIMPLEX VIRUS
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批准号:6104740
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资助金额:$12.07万
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财政年份:1998
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负责人:GARY H COHEN
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依托单位:
CORE--BACULOVIRUS
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批准号:6273814
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项目类别:
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资助金额:$16.7万
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财政年份:1998
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负责人:GARY H COHEN
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依托单位:
DETERMINATION OF OLIGOMERIC STATE OF HSV GLYCOPROTEINS GD, GH & GL, USING STEM
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批准号:6281336
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项目类别:
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资助金额:$1.06万
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财政年份:1998
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负责人:GARY H COHEN
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依托单位:
DETERMINATION OF OLIGOMERIC STATE OF HSV GLYCOPROTEINS G USING STEM
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批准号:6251685
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资助金额:$0.84万
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财政年份:1997
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负责人:GARY H COHEN
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依托单位:
FUNCTIONAL ANALYSIS OF GLYCOPROTEIN GD OF HERPES SIMPLEX VIRUS
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批准号:6270290
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项目类别:
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资助金额:$28.12万
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财政年份:1997
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负责人:GARY H COHEN
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依托单位:
FUNCTIONAL ANALYSIS OF GLYCOPROTEIN GD OF HERPES SIMPLEX VIRUS
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项目类别:
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资助金额:$27.3万
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负责人:GARY H COHEN
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依托单位:
STUDIES OF HERPES SIMPLEX VIRUS GLYCOPROTEINS
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批准号:2060671
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项目类别:
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资助金额:$19.98万
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财政年份:1981
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负责人:GARY H COHEN
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依托单位:
STUDIES OF HERPES SIMPLEX VIRUS GLYCOPROTEINS
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批准号:3127810
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项目类别:
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资助金额:$19.14万
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负责人:GARY H COHEN
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依托单位:
STUDIES OF GLYCOPROTEIN D OF HERPES SIMPLEX VIRUS
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批准号:3127814
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项目类别:
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资助金额:$17.51万
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财政年份:1981
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负责人:GARY H COHEN
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依托单位:
Studies of Herpes Simplex Virus Glycoproteins
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批准号:8282650
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项目类别:
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资助金额:$39.03万
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财政年份:1981
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国内基金
海外基金
Journal of Genetics and Genomics
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批准号:31224803
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资助金额:24.0万元
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批准年份:2012
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依托单位: