3D TOMOGRAPHY MODELS IN ELECTRON MICROSCOPY OF CRYSTALS

晶体电子显微镜中的 3D 断层扫描模型

• 批准号: 6472789
• 负责人: PABLO SALZBERG
• 金额: $ 11.03万
• 依托单位: UNIVERSITY OF PUERTO RICO RIO PIEDRAS
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6472789
• 关键词: X ray crystallography; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment; computed axial tomography; computer program /software; electron crystallography; image processing; mathematical model; model design /development

Description (Adapted from Application): This research proposal deals with the development of two highly parallelizable, fully three-dimensional reconstruction models with extensive applicability in the field of electron microscopy (EM) of crystals, based on the geometry of the 3D Torus. One of the imaging models proposed is aimed at the study of the atomic structure of crystals formed by atoms of more than one kind, by means of transmission EM. The present work is an enhancement to the previous successful model developed specifically for tomography of crystals endowed with a single kind of atom. The other tomography model allows the imaging of crystallized biological molecules and molecular aggregates and complexes, such as RNA, DNA, proteins and ribosomes, which can be crystallized. This work is fundamental in that it could permit the extraction of previously unobtainable structural and morphological information from the electron microscopy of biomolecules. Due to a robust signal-to-noise ratio, this reconstruction technique offers an additional advantage by requiring lower radiation doses than conventional methods, which could guarantee sample integrity by limiting the structural degradation in complex organic species. A comparative study of the quality and speed of the reconstruction will be performed on data obtained from crystallized specimens of current interest, taking into consideration the performance of other existing imaging models. As a byproduct of the proposed research, the investigators expect to produce software packages to be used in EM facilities, for both models. The long-term objective of this proposal is to create an image processing unit at the University of Puerto Rico, integrated to a multidisciplinary environment, aimed at assisting the needs of our researchers in the biomedical area.

描述（改编自申请）：本研究计划涉及 开发两个高度并行的，完全三维的 在电子领域具有广泛适用性的重建模型 基于3D Torus的几何形状，晶体的显微镜（EM）。之一 成像模型的目的是研究的原子结构， 通过透射电镜观察由一种以上的原子形成的晶体。 目前的工作是一个增强到以前的成功模型开发 特别是用于具有单一种类原子的晶体的层析成像。的 其它层析成像模型允许对结晶的生物分子成像 以及分子聚集体和复合物，如RNA、DNA、蛋白质和 核糖体，可以结晶。这项工作的基础在于它可以 允许提取以前无法获得的结构和形态 生物分子的电子显微镜信息。由于强大的 信噪比，这种重建技术提供了一个额外的 通过比传统方法需要更低的辐射剂量， 可以通过限制样品中的结构降解来保证样品的完整性， 复杂的有机物种。 将对重建的质量和速度进行比较研究， 对从当前感兴趣的结晶样品获得的数据进行， 考虑到其他现有成像模型的性能。作为 作为这项研究的副产品，研究人员希望能产生 两种型号的EM设施中使用的软件包。长期 该提案的目的是在该区域建立一个图像处理单元， 波多黎各大学，整合到一个多学科的环境，旨在 帮助我们的生物医学领域研究人员的需求。