ACID TOLERANCE/MICROBIAL ECOLOGY--E COLI 0157:H7 IN RUME

耐酸性/微生物生态学--酸模中的大肠杆菌 0157:H7

基本信息

  • 批准号:
    6469240
  • 负责人:
  • 金额:
    $ 6.87万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2001
  • 资助国家:
    美国
  • 起止时间:
    2001-06-01 至 2002-05-31
  • 项目状态:
    已结题

项目摘要

E. coli O157:H7 is an important pathogen derived from animal products or vegetables treated with animal waste. One of the factors that enables this organism to survive the stomach and colonize the large intestine of humans is its ability to tolerate acidic conditions. The mechanisms that confer acid tolerance are unclear, but probably colonization inhibition could be developed. Our overall goal for this proposal is to understand the role of acid 1) optimization of procedures for extracting E. coli O157:H7 DNA from bovine ruminal fluid and feces, 2) a 'shock' is necessary to induce the tolerance, 3) determination of the possible roles for rpoS (and other acid resistance systems) and anaerobiosis in allowing E. coli 0157:H7 to compete with microorganisms from the O157:H7 and ruminal lactate-utilizing bacteria as well as ruminal lactate-producing bacteria as well as ruminal lactate-producing bacteria. Our working hypothesis is that organisms that utilize lactate can assist in decreasing carriage of E. coli 0157:H7. It has been suggested that low ruminal pH of cattle consuming corn-based diets induces acid tolerance in E. coli O157:H7. However, this organism is capable of surviving in manure for long periods of time under environmental conditions. Thus, acid resistance may already be induced. The most promising plan of action appears to be reduction of E. coli O157:H7 carriage in cattle. Development of probiotic organisms that are indigenous to the rumen ecosystem holds the greatest potential for success. Thus, the long-term objective for our research is to develop probiotic treatment based on organisms indigenous to the rumen environment. The outcome of this study will assist others in future applications of this organism in management of infection by cattle, and hopefully in the protection of the food supply. By employing a host of techniques including anaerobic microbiology, PCR amplification, and hybridization, we are confident that we can realize the aims in this proposal within four years.
大肠杆菌O157:H7是一种重要的病原体,来源于用动物粪便处理过的动物产品或蔬菜。使这种生物能够在胃中存活并在人类大肠中定居的因素之一是它耐受酸性条件的能力。赋予耐酸性的机制尚不清楚,但可能可以开发定殖抑制。我们的总体目标是了解酸的作用:1)优化从牛瘤胃液和粪便中提取大肠杆菌O157:H7 DNA的程序;2)“休克”是诱导耐受性的必要条件;3)确定rpoS(和其他耐酸系统)和厌氧作用在允许大肠杆菌0157:H7与来自O157:H7和瘤胃乳酸利用菌、瘤胃乳酸产生菌以及瘤胃乳酸产生菌的微生物竞争中的可能作用。我们的工作假设是,利用乳酸的生物体可以帮助减少大肠杆菌0157:H7的携带。有研究表明,食用玉米基饲粮的牛的瘤胃pH值较低可诱导大肠杆菌O157:H7的耐酸性。然而,在环境条件下,这种生物能够在粪便中长时间生存。因此,耐酸性可能已经被诱导。最有希望的行动计划似乎是减少牛中携带O157:H7大肠杆菌。瘤胃生态系统中原生益生菌的开发具有最大的成功潜力。因此,我们研究的长期目标是开发基于瘤胃环境原生生物的益生菌治疗。这项研究的结果将有助于今后将这种微生物应用于牛感染的管理,并有望保护食品供应。通过采用厌氧微生物学、PCR扩增和杂交等一系列技术,我们有信心在四年内实现本提案的目标。

项目成果

期刊论文数量(0)
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TAMMY MAY其他文献

TAMMY MAY的其他文献

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{{ truncateString('TAMMY MAY', 18)}}的其他基金

ACID TOLERANCE/MICROBIAL ECOLOGY--E COLI 0157:H7 IN RUME
耐酸性/微生物生态学--酸模中的大肠杆菌 0157:H7
  • 批准号:
    6608641
  • 财政年份:
    2002
  • 资助金额:
    $ 6.87万
  • 项目类别:
ACID TOLERANCE/MICROBIAL ECOLOGY--E COLI 0157:H7 IN RUME
耐酸性/微生物生态学--酸模中的大肠杆菌 0157:H7
  • 批准号:
    6584145
  • 财政年份:
    2002
  • 资助金额:
    $ 6.87万
  • 项目类别:
ACID TOLERANCE/MICROBIAL ECOLOGY--E COLI 0157:H7 IN RUME
耐酸性/微生物生态学--酸模中的大肠杆菌 0157:H7
  • 批准号:
    6506278
  • 财政年份:
    2001
  • 资助金额:
    $ 6.87万
  • 项目类别:
ACID TOLERANCE/MICROBIAL ECOLOGY--E COLI 0157:H7 IN RUME
耐酸性/微生物生态学--酸模中的大肠杆菌 0157:H7
  • 批准号:
    6316653
  • 财政年份:
    2000
  • 资助金额:
    $ 6.87万
  • 项目类别:
ISOLATION AND CHARACTERIZATION OF RUMINAL PROTEASES
瘤胃蛋白酶的分离和表征
  • 批准号:
    6107208
  • 财政年份:
    1999
  • 资助金额:
    $ 6.87万
  • 项目类别:
ISOLATION AND CHARACTERIZATION OF RUMINAL PROTEASES
瘤胃蛋白酶的分离和表征
  • 批准号:
    6271601
  • 财政年份:
    1998
  • 资助金额:
    $ 6.87万
  • 项目类别:
ISOLATION AND CHARACTERIZATION OF RUMINAL PROTEASES
瘤胃蛋白酶的分离和表征
  • 批准号:
    6240108
  • 财政年份:
    1997
  • 资助金额:
    $ 6.87万
  • 项目类别:
ISOLATION AND CHARACTERIZATION OF RUMINAL PROTEASES
瘤胃蛋白酶的分离和表征
  • 批准号:
    5211723
  • 财政年份:
  • 资助金额:
    $ 6.87万
  • 项目类别:
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